Turn-on split fluorescent tag responding to enzymatic ligation
Turn-on split fluorescent tag responding to enzymatic ligation
批准号:
23656518
负责人:
YAMAGUCHI Satoshi
金额:
$2.5万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
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英文摘要
The labeling method consisting of a peptide tag and a fluorescent dye which can specifically label a target protein and turn on the fluorescence after labeling was developed. We found the pair of tag sequences and a dye which fluorescent intensities were increased 2.6 times after specific labeling via soltase-mediated ligation. Furthermore, to enable intracellular labeling, a method for intracellular specific ligation was successfully developed by transducing Streptococcus pyogenes sortase A into living mammalian cells.
期刊论文(12)
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酵素反応でturn-onするスプリット蛍光タグの開発
开发通过酶反应开启的分裂荧光标签
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Jinhua Dong, Akira Sakurai, Namiko Nomura, Enoch Y.Park, Futoshi Shibasaki, Hiroshi Ueda, 山口哲志,井上紫織,平川秀彦,長棟輝行]
通讯作者:
山口哲志,井上紫織,平川秀彦,長棟輝行
Photo-assisted protein transduction into cytoplasm by modifying or mixing with fluorophore-conjugated poly-arginine peptides
通过修饰或与荧光团缀合的聚精氨酸肽混合,光辅助蛋白质转导至细胞质
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Y. Maeda, S. Yamaguchi, W. Ishihara, H.Hirakawa, T. Nagamune]
通讯作者:
T. Nagamune
光応答性分子修飾による蛋白質の細胞内局在制御
通过光响应分子修饰控制蛋白质的细胞内定位
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[桑原泰隆, 山下弘巳, 前田泰一,山口哲志,長棟輝行]
通讯作者:
前田泰一,山口哲志,長棟輝行
Intracellular protein cyclization catalyzed by streptococcus pyogenes sortase A through protein transduction
化脓性链球菌分选酶 A 通过蛋白质转导催化细胞内蛋白质环化
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[J. Zhang, S. Yamaguchi, H. Hirakawa, T.Nagamune]
通讯作者:
T.Nagamune
Enzymatic protein labeling using turn-on split fluorescent tag
使用开启分裂荧光标签进行酶促蛋白质标记
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[J. Zhang, S. Yamaguchi, S. Inoue, H.Hirakawa, T. Nagamune]
通讯作者:
T. Nagamune
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