Regulating cell function by introduction of the proteins enzymatically modified with synthetic functional molecules into cell membrane or cytoplasm

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We developed technologies that can transfer target proteins into cellular membrane and cytoplasm by enzymatically-specific modification of the C-terminal of target proteins with synthetic functional molecules. By the present technologies、target proteins could be modified with PEG-lipid on cellular membrane. This method realized that the efficiency in the phagocytosis of dendritic cells for cancer cells was successfully enhanced by introducing antibodies onto cancer membranes. In addition、we developed a method that can easily transfer target proteins to cytoplasm by attaching cell-penetrating peptide bearing the dye、which generates radical under photo-irradiation、to target protein and irradiating light after cellular uptake of the present modified protein.