Bone regeneration by BMP2-gene transduced mesenchymal stem cells.

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The goals of this project is regeneration of bone by autotransplantation of osteoblastic cells derived from mesenchymal stem cells in bone marrow. We have performed studies as follows.(1)Bone regeneration by BMP2-gene transduced mesenchymal stem cellsWe generated recombinant retrovirus vector including human BMP2 cDNA. Primary cultured rat mesenchymal stem cells were differentiated into osteoblasts by retrovirus mediated gene transduction of BMP2. Histological analysis showed that the newly bone formation in vivo by transplantation of BMP2-transduced bone marrow stem cells seeded on hydroxyapatite.(2)Novel scaffold for bone regenerationWe produced a poly (ethylene glycol)(PEG) hydrogel cross-linked by a hydrolyzable polyrotaxane containing hydroxyapatite particles (PRX-HAp). Osteoblasts were observed to attach and survive on the surface of PRX-HAp. Histological analysis showed that osteoid-like tissues were seen in the region between PRX-HAps and a queue of osteoblast-like cells. The PRX-HAps have the great potential for bone tissue engineering.(3)Expansion of human bone marrow stromal cells with patient's autologus serumTo overcome the risk of bovine serum, we examined whether a patient's autologous serum could support the growth and differentiation of his/her bone marrow stromal cells (BMSCs). A patient's autologous serum could expand BMSCs without losing their potentiality for osteoblastic differentiation. Patients' autologous serum could be efficient to expand BMSCs and to be utilized safely for bone regeneration therapy.