Signal transduction of GPI-anchored proteins in lipid rafts

脂筏中 GPI 锚定蛋白的信号转导

• 批准号: 13680701
• 负责人: KASAHARA Kohji
• 金额: $ 1.34万
• 依托单位: Tokyo Metropolitan Institute of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680701/
• 关键词: glycosphingolipids; raft; GPI-anchor; signal transduction

The mechanism of signal transduction by GPI-anchored neural cell adhesion molecules is obscure, because GPI-anchored proteins have no transmembrane domains. Recent studies suggest dynamic clustering of glycosphingolipids (lipid rafts) function as platforms for attachment of GPI-anchored proteins, src-family kinases during signal transduction. In this research project, I pursued clarifying mechanism of signal transduction by GPI-anchored neural cell adhesion molecules.I previously reported that GPI-anchored neural cell adhesion molecule TAG-1 transduces signal via src-family kinase Lyn in lipid rafts of rat primary cerebellar cultures. In this year, I found that phosphacan, TAG-1 ligand, induces signal transduction in lipis rafts via src-family kinase Lyn and has repulsive effects on rat primary cerebellar cultures.

GPI锚定的神经细胞粘附分子的信号转导机制尚不清楚，因为GPI锚定蛋白没有跨膜结构域。最近的研究表明，动态聚集的鞘糖脂（脂筏）的功能作为平台的GPI锚定蛋白，src家族激酶在信号转导过程中的附件。在本研究项目中，我致力于阐明GPI锚定的神经细胞粘附分子的信号转导机制，我以前报道过GPI锚定的神经细胞粘附分子TAG-1通过src家族激酶林恩在大鼠原代小脑培养的脂筏中进行信号转导。在这一年中，我发现phosphacan，TAG-1的配体，通过src-家族激酶林恩诱导脂筏中的信号转导，并对大鼠原代小脑培养物具有排斥作用。

项目成果

Kasahara K, Sanai Y: "Functional roles of glycoconjugates in signal transduction via lipid rafts"Trend. Glycosci. Glycotech.. 13(71). 251-259 (2001)

Kasahara K、Sanai Y：“糖复合物在脂筏信号转导中的功能作用”趋势。

Kasahara K, et al.: "Association of GPI-anchored protein TAG-1 with src-family kinase lyn in lipid rafts of cerebellar granule cells"Neurochemical Resesrch. 27. 823-829 (2002)

Kasahara K 等人：“小脑颗粒细胞脂筏中 GPI 锚定蛋白 TAG-1 与 src 家族激酶 lyn 的关联”神经化学研究。

Yamauchi et al.: "Phosphorylation of neuroglycan C, a brain-specific transmembrane chondroitin sulfate"J.Biol.Chem.. 277. 20583-20590 (2002)

Yamauchi 等人：“神经聚糖 C 的磷酸化，一种脑特异性跨膜硫酸软骨素”J.Biol.Chem.. 277. 20583-20590 (2002)

Yamauchi S, Tokita Y, Aono S, Matsui F, Shuo T, Ito H, Kato K, Kasahara K, and Oohira A: "Phosphorylation of neuroglycan C, a brain-specific transmembrane chondroitin sulfate proteoglycan and its localization in the lipid rafts"J.Biol.Chem.. 277. 20583-20

Yamauchi S、Tokita Y、Aono S、Matsui F、Shuo T、Ito H、Kato K、Kasahara K 和 Oohira A：“神经聚糖 C 的磷酸化，一种脑特异性跨膜硫酸软骨素蛋白聚糖及其在脂筏中的定位”

笠原浩二, 佐内豊: "神経系細胞膜ラフトとシグナル伝達"蛋白質核酸酵素. 47. 333-337 (2002)

Koji Kasahara、Yutaka Sanai：“神经系统细胞膜筏和信号转导”蛋白质核酸酶 47. 333-337 (2002)。