Phylogenetic relationships and divergence times of reptiles inferred by mitochondrial DNA sequences

通过线粒体DNA序列推断爬行动物的系统发育关系和分化时间

• 批准号: 14540641
• 负责人: KUMAZAWA Yoshinori
• 金额: $ 2.62万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540641/
• 关键词: lizards; snakes; mitochondrial DNA; molecular phylogeny; divergence time; gene organization; vicariance; biogeography; 爬虫類; PCR; 分子系統解析; 高次系統分類; 系統樹

During the granted period, we have sequenced mitochondrial DNAs of various reptiles. These data have been characterized in both the evolution of mitochondrial genome organization and the pattern and timing of phylogenetic divergences. We found that mtDNAs of some reptile groups (e.g., snakes, varanids, agamids, and cordylids) have distinct gene organizations from the typical organization found in most vertebrates. We then addressed when and how these gene rearrangements took place on ancestral lineages of each group by comparing sequences of the corresponding mtDNA region among relevant taxa. With respect to phylogenetic matters, the following implications were obtained. 1) Lacertilia can be divided into four groups mostly corresponding to infraorders suggested by morphological studies, 2) Amphisbaenia is nested within Lacertilia and is the most closely related to Lacertidae, 3) Among the four lizard infraorders, Gekkota rather than Iguania diverged first, 4) Snakes are not closely related to Varanoidea, and 5) Lizard families have deep divergences extending to Triassic and Jurassic.

在授权期内，我们已经对各种爬行动物的线粒体DNA进行了测序。这些数据在线粒体基因组组织的进化以及系统发育分化的模式和时间上都有特征。我们发现，一些爬行动物群体(如蛇、瓦拉尼德、阿格米德和虫草)的mtDNA具有与大多数脊椎动物中典型组织不同的基因组织。然后，我们通过比较相关类群中相应mtDNA区域的序列，研究了这些基因重排何时以及如何在每个群体的祖先谱系上发生。关于系统发育问题，获得了以下影响。1)蜥蜴类可分为4个类群，主要对应于形态研究提出的下位亚目；2)两栖动物嵌套在下位亚目内，与蜥科的亲缘关系最近；3)在4个下位亚目中，壁虎而不是蜥蜴最先分化；4)蛇类与蜥蜴总科的亲缘关系不大；5)蜥蜴科的分化一直延伸到三叠纪和侏罗纪。

项目成果

Miya, M.et al.: "Major patterns of higher teleostean phylogenies : a new perspective based on 100 complete mitochondrial DNA sequences"Molecular Phylogenetic and Evolution. 26. 121-138 (2003)

Miya, M.等人：“高等硬骨动物系统发育的主要模式：基于 100 个完整线粒体 DNA 序列的新视角”分子系统发育和进化。

Dynamics of the Ocean Biosystems, vol.1. "Chapter 7 : Divergence time estimation for fishes using molecular data." (Mutsumi Nishida (ed))

海洋生物系统动力学，第一卷。

• 作者: Kumazawa;Y.;熊澤慶伯;Kumazawa Y.
• 通讯作者: Kumazawa Y.

Tempo of mitochondrial gene evolution : can mitochondrial DNA be used to date old divergences?

线粒体基因进化的节奏：线粒体 DNA 可以用来确定古老的分歧吗？

• 发表时间: 2004
• 期刊: Endocytobiosis Cell research 15
• 作者: IKUMAZAWA;Yoshinori
• 通讯作者: Yoshinori

The mitochondrial genome of Indonesian coelacanth Latimeria menadoensis (Coelacant hiformes) and divergence time estimation between the two coelacanths.

印度尼西亚腔棘鱼 Latimeria menadoensis (Coelacant hiformes) 的线粒体基因组和两种腔棘鱼之间的分歧时间估计。

• 发表时间: 2005
• 期刊: Gene (In press)
• 作者: Inoue;J.G.;Miya;M.;Venkatesh;B.;Nishida;M.
• 通讯作者: M.

Kumazawa, Y., Endo, H.: "Mitochondrial genome of the Komodo dragon : efficient sequencing method with reptile-oriented primers and novel gene rearrangements"DNA Research. (in press). (2004)

Kumazawa, Y., Endo, H.：“科莫多巨蜥的线粒体基因组：利用爬行动物导向引物和新基因重排的高效测序方法”DNA 研究。