Study of brain plasticity and functional and psychological adaptability caused by alteration of oral environment

口腔环境改变引起的脑可塑性及功能心理适应性研究

• 批准号: 15390587
• 负责人: WATANABE Makoto
• 金额: $ 6.66万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390587/
• 关键词: functional MRI; brain plasticity; oral environment; oral function; functional MRI

We evaluated brain function using functional magnetic resonance image (fMRI) to demonstrate brain activity change of mastication. fMRI acquisition protocol used in the present study has been optimised in our pilot study. 6 adult male subjects (mean age 23.2) were told to use occlusal splint for 7 days. fMRI was performed on day 1, 3, 7 of occlusal splint use and day 7 after occlusal splint removal. After fMRI acquisition, subjective sensations of every subject were recorded with "Visual Analog Scale". fMRI data were analyzed with SPM2 (Statistical Parametric Mapping 2) to determine differences of brain activity between with and without occlusal Splint (p=0.001 uncorrected, extent threshold=20voxels). On day 1 of occlusal splint use, cerebellum and left middle frontal gyrus (BA10) were more activated with occlusal splint than without it. These results showed the activation of cerebellum and frontal lobe during "motor learning". On day 3 and 7 of occlusal splint use, no difference of activity was detected in cerebellum and frontal lobe. On day 7 after occlusal splint removal, left premotor cortex was more activated with occlusal splint than without it. These findings suggest that brain neural network corresponding to new pattern of masticatory movement was constructed and maintained after occlusal splint removal. Although we analyzed association between subjective sensation score by VAS and brain activity using SPM2, no significant correlation was observed.

采用功能磁共振成像（fMRI）技术，观察咀嚼运动时脑功能的变化.本研究中使用的fMRI采集协议已在我们的试点研究中进行了优化。6名成年男性受试者（平均年龄23.2岁）被告知使用咬合板7天。分别于使用咬合板后第1、3、7天及取出咬合板后第7天进行fMRI检查。采集功能磁共振图像后，用视觉模拟量表记录受试者的主观感觉。使用SPM 2（统计参数映射2）分析fMRI数据，以确定使用和不使用咬合夹板之间的脑活动差异（p=0.001未校正，范围阈值= 20体素）。在使用咬合板的第1天，小脑和左侧额中回（BA10）在使用咬合板的情况下比不使用咬合板的情况下激活更多，表明小脑和额叶在“运动学习”过程中的激活。在使用咬合板的第3天和第7天，小脑和额叶的活动没有差异。结果表明，在去除咬合板后第7天，左侧运动前区皮层的激活程度明显高于无咬合板时，提示在去除咬合板后，与新的咀嚼运动模式相对应的脑神经网络得以建立和维持。尽管我们分析了VAS主观感觉评分与SPM 2脑活动之间的相关性，但未观察到显著相关性。

项目成果

Occlusal and TMJ Loads in Subjects with Experimentally Shortened Denral Arches.

实验性缩短牙弓受试者的咬合和颞下颌关节负荷。

• 发表时间: 2003
• 期刊: J Dent Res. 82(7)
• 作者: Hattori Y;Satoh C;Seki S;Watanabe Y;Ogino Y;Watanabe M
• 通讯作者: Watanabe M

Change of Occlusal Contact Areas Following the Cure of Temporomandibular Disorders.

颞下颌疾病治愈后咬合接触区域的变化。

• 发表时间: 2004
• 期刊: Dentistry in Japan. 40
• 作者: Tsuboi A;Tanabe T;Watanabe M
• 通讯作者: Watanabe M

Occlusal and TMJ loads in subjects with experimentally shortened dental arches

• DOI: 10.1177/154405910308200709
• 发表时间: 2003-07-01
• 期刊: JOURNAL OF DENTAL RESEARCH
• 影响因子: 7.6
• 作者: Hattori, Y;Satoh, C;Watanabe, M
• 通讯作者: Watanabe, M

Cloning, characterization and expression of two alternatively splicing isoforms of Ca2+/calmodulin‐dependent protein kinase Iγ in the rat brain

• DOI: 10.1046/j.1471-4159.2003.01760.x
• 发表时间: 2003-06
• 期刊: Journal of Neurochemistry
• 影响因子: 4.7
• 作者: H. Nishimura;H. Sakagami;Akiyoshi Uezu;K. Fukunaga;Makoto Watanabe;H. Kondo

Overall activity of all masticatory muscles during lateral excursion.

横向移动期间所有咀嚼肌的总体活动。

• 发表时间: 2006
• 期刊: J Dent Res. 85(1)
• 作者: Yamaguchi S;Rikimaru H;Yamaguchi K;Itoh M;Watanabe M
• 通讯作者: Watanabe M