Elucidation of the cause of Caribbean parkinsonism and prevention in other area

在瓜德罗普岛，法属西印度群岛，非典型帕金森病或进行性核上性麻痹的发病率很高，所有被调查的患者都服用凉茶或番石榴科的热带水果。当地居民吃这种水果，也喝由叶子制成的茶。本研究采用液相色谱-串联质谱（LC/MS/MS）联用多反应监测（MRM）技术对番荔枝科植物中低分子神经毒性苯并异喹啉衍生物进行检测。除了果肉和种子外，我们在每一个被检测的番荔枝样品中都检测到网状碱和n -甲基古兰嘌呤。在番荔枝果实中未检测到它们。未检出去甲网碱。这三种化合物对SH-SY5Y神经母细胞瘤细胞有毒性，并抑制线粒体呼吸复合体i。可能苯基异喹啉衍生物网嘌呤和n -甲基环嘌呤的摄取及其在脑中的积累可能与局部地方性疾病的发病机制有关。

In Guaderoupe, the French West Indies, there is a high incidence of atypical parkinsonism or progressive supranuclear palsy, and all of the investigated patients had taken herbal tea or tropical fruits of the Annonaceae family. Local inhabitants consume the fruits, and also drink tea made from the leaves. In the present study, we used liquid chromatography-tandem mass spectrometry (LC/MS/MS) with multiple reaction monitoring (MRM) to detect low-molecular-weight neurotoxic benzylisoquinoline derivatives in the Annonaceae family. We detected reticuline and N-methylcoculaurine in every Annona muricata sample examined, except for pulp and seed. They were not detected in sweetsop fruits. Norreticuline was not detected in any sample. These three compounds were toxic to SH-SY5Y neuroblastoma cells and inhibited mitochondrial respiratory complex I. It is possible that uptake of the benzylisoquinoline derivatives reticuline and N-methylcoculaurine and their accumulation in the brain may be related to the pathogenesis of the local endemic disease.