STUDY ON THE EXPRESSION OF ESTROGEN RECEPTORS IN MALIGNANT RHABDOID TUMOR

恶性横纹肌瘤中雌激素受体表达的研究

• 批准号: 14570741
• 负责人: OHTA Shigeru
• 金额: $ 1.09万
• 依托单位: SHIGA UNIVERSITY OF MEDICAL SCIENCE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570741/
• 关键词: Malignant rhabdoid tumor; estrogen; anti-estrogen; tamoxifen; apoptosis; MRT; estrogen receptor; 悪性横紋筋様腫瘍; Malignant rhabdoid tumor

Expression of estrogen receptors and cytotoxic effects of the anti-estrogens on malignant rhabdoid tumor (MRT) cell lines were examined.Materials and Methods: Six cell lines of MRT(TM87-16, STM91-01, TTC549, TTC642, TTC1240 and YAM-RTK1) were used for the examination on the expression of estrogen receptor alpha(ERα), progesterone receptor which has some correlation to estrogen, and pS2. The effect of Tamoxifen(TAM) on the cell proliferation of MRT cell lines expressing ERα was examined. TAM has E2(estrogen) as well as anti-E2 effects. So the effect of ICI, which is a pure anti-estrogen, on the cellular proliferation of MRT cell lines was also checked.Results: The expression of ER-α was detected in TTC87-16, TTC642 and TTC1240. The expression of pS2 was not seen. The proliferation of these MRT cells was arrested with the addition of TAM and apoptosis was induced. Inhibition of cellular proliferation was note induced with ICI. The suppression of cellular proliferation was note improved with the simultaneous administration of TAM and E2.Discussion: Some of MRT cell lines expressed ER-α and showed the inhibition of cellular growth with TAM. This effect was delivered from the pathway other than ER-α. There will be a possible use of TAM as a agent for tumor dormancy therapy for MRT.

检测恶性横纹肌样瘤（MRT）细胞系雌激素受体的表达和抗雌激素的细胞毒作用。材料和方法：采用6株MRT细胞系（TM 87 -16、STM 91 -01、TTC 549、TTC 642、TTC 1240和YAM-RTK 1）检测雌激素受体α（ERα）、与雌激素有一定相关性的孕激素受体和pS2的表达。观察了三苯氧胺（TAM）对表达ERα的MRT细胞株增殖的影响。TAM具有E2（雌激素）和抗E2作用。结果：TTC 87 -16、TTC 642和TTC 1240细胞中均检测到ER-α的表达，而TTC 1240细胞中ER-α的表达量明显低于ICI。pS2蛋白表达阴性。加入TAM后，这些MRT细胞的增殖被抑制，并诱导凋亡。细胞增殖的抑制被ICI诱导。TAM和E2联合应用可明显改善对细胞增殖的抑制作用。讨论：部分MRT细胞表达ER-α，TAM可抑制细胞生长。这种作用是通过ER-α以外的途径传递的。将有一个可能的使用TAM作为一个代理的肿瘤休眠治疗MRT。

项目成果

太田 茂, 成田 努, 他: "Rhabdoid Tumor -基礎研究の最新知見"小児外科. 34. 440-445 (2002)

Shigeru Ota、Tsutomu Narita 等人：“横纹肌样肿瘤 - 基础研究的最新发现”小儿外科。 34. 440-445 (2002)

Shinobu Yoshida, Shigeru Ohta et al.: "TRAIL/Apo2L Ligands induce apoptosis in Malignant Rhabdoid Tumor cell lines."Pediatric Research. 54巻5号. 709-717 (2003)

Shinobu Yoshida、Shigeru Ohta 等人：“TRAIL/Apo2L 配体诱导恶性横纹肌瘤细胞系凋亡。”儿科研究，第 54 卷，第 5 期。709-717（2003 年）

太田 茂, 成田 努, 他: "Rhabdoid Tumor...基礎研究の最新知見"小児外科. 34・4. 440-445 (2002)

Shigeru Ota、Tsutomu Narita 等：“横纹肌样肿瘤……基础研究的最新发现”34・4（2002 年）。

Hirofumi Kato, Shigeru Ohta, et al.: "Expression of pericyte, mesangium and muscle markers in malignant rhabdoid tumor cell lines: Differentiation induction using 5-azacytidine"Cancer Science. 94:(10). 1059-1065 (2003)

Hirofumi Kato、Shigeru Ohta 等人：“恶性横纹肌样肿瘤细胞系中周细胞、系膜和肌肉标记物的表达：使用 5-氮杂胞苷进行分化诱导”癌症科学。

Shigeki Koshida, Shigeru Ohta, et al.: "Estrogen receptor expression and estrogen receptor-independent cytotoxic effects of tamoxifen on malignant rhabdoid tumor cells in vitro"Japanese Journal of Cancer Research. 163. 1351-1357 (2002)

Shigeki Koshida、Shigeru Ohta 等人：“体外雌激素受体表达和他莫昔芬对恶性横纹肌样肿瘤细胞的雌激素受体非依赖性细胞毒性作用”日本癌症研究杂志。