STUDY ON THE EXPRESSION OF ESTROGEN RECEPTORS IN MALIGNANT RHABDOID TUMOR

检测恶性横纹肌样瘤（MRT）细胞系雌激素受体的表达和抗雌激素的细胞毒作用。材料和方法：采用6株MRT细胞系（TM 87 -16、STM 91 -01、TTC 549、TTC 642、TTC 1240和YAM-RTK 1）检测雌激素受体α（ERα）、与雌激素有一定相关性的孕激素受体和pS2的表达。观察了三苯氧胺（TAM）对表达ERα的MRT细胞株增殖的影响。TAM具有E2（雌激素）和抗E2作用。结果：TTC 87 -16、TTC 642和TTC 1240细胞中均检测到ER-α的表达，而TTC 1240细胞中ER-α的表达量明显低于ICI。pS2蛋白表达阴性。加入TAM后，这些MRT细胞的增殖被抑制，并诱导凋亡。细胞增殖的抑制被ICI诱导。TAM和E2联合应用可明显改善对细胞增殖的抑制作用。讨论：部分MRT细胞表达ER-α，TAM可抑制细胞生长。这种作用是通过ER-α以外的途径传递的。将有一个可能的使用TAM作为一个代理的肿瘤休眠治疗MRT。

Expression of estrogen receptors and cytotoxic effects of the anti-estrogens on malignant rhabdoid tumor (MRT) cell lines were examined.Materials and Methods: Six cell lines of MRT(TM87-16, STM91-01, TTC549, TTC642, TTC1240 and YAM-RTK1) were used for the examination on the expression of estrogen receptor alpha(ERα), progesterone receptor which has some correlation to estrogen, and pS2. The effect of Tamoxifen(TAM) on the cell proliferation of MRT cell lines expressing ERα was examined. TAM has E2(estrogen) as well as anti-E2 effects. So the effect of ICI, which is a pure anti-estrogen, on the cellular proliferation of MRT cell lines was also checked.Results: The expression of ER-α was detected in TTC87-16, TTC642 and TTC1240. The expression of pS2 was not seen. The proliferation of these MRT cells was arrested with the addition of TAM and apoptosis was induced. Inhibition of cellular proliferation was note induced with ICI. The suppression of cellular proliferation was note improved with the simultaneous administration of TAM and E2.Discussion: Some of MRT cell lines expressed ER-α and showed the inhibition of cellular growth with TAM. This effect was delivered from the pathway other than ER-α. There will be a possible use of TAM as a agent for tumor dormancy therapy for MRT.