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Radiobiological and Molecular Biological Study of Enhancement Effect of High LET Heavy Ion Irradiation and Chemotherapeutic Agents in vitro

高LET重离子照射与化疗药物体外增强作用的放射生物学和分子生物学研究

基本信息

批准号：
17591292
负责人：
TAKAHASHI Takeo
金额：
$ 1.95万
依托单位：
Saitama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591292/
关键词：
radiosensitivity heavy ion irradiation anticancer drug apoptosis synergistic effect cell cycle DNA repair

项目摘要

We investigate the differences between two rat yolk sac tumor cell lines with different radiosensitivities in sensitivity to high-LET heavy ion beam and in sensitizing effect and the mechanism of cisplatin and etoposide in combination with heavy-ion beam. NMT-1 (wild-type p53 cell) is a parent radiosensitive cell line and NMT-1R (mutant-type p53 cell) is a variant radioresistant cell line. The dose average LET value in the samples was 80 keV/μm. The concentration of chemotherapeutic agents required to reduce colony formation by 50% at 1-hour treatment (IC50 of etoposide) was selected for heavy ion irradiation pretreatment for each cell line. The mechanism of the enhancement effect was investigated by apoptosis and cell cycle and mechanism of DNA repair. The RBE of carbon beam was larger in mutant-type p53 cells than in wild-type p53 cells. Etoposide showed a synergistic effect in combination with carbon beam irradiation in NMT-1R cells. However, there was no enhancement effect in radiosensitive NMT-1 cells. The incidence of apoptosis, the distribution of cell cycle and the ability of DNA repair did not change between carbon beam irradiation alone and the combination of carbon beam irradiation and etoposide od cisplation in both cell lines.

本研究探讨了两种不同放射敏感性的大鼠卵黄囊瘤细胞系对高LET重离子束的敏感性和增敏效应的差异，以及顺铂和足叶乙甙联合重离子束的增敏机制。NMT-1（野生型p53细胞）是亲本放射敏感细胞系，NMT-1 R（突变型p53细胞）是变异放射抗性细胞系。样品中的剂量平均LET值为80 keV/μm。选择在1小时处理时使集落形成减少50%所需的化疗剂浓度（依托泊苷的IC 50）用于每个细胞系的重离子辐照预处理。从细胞凋亡、细胞周期、DNA修复机制等方面探讨其作用机制。碳束对突变型p53细胞的RBE大于野生型p53细胞。依托泊苷与碳束照射联合应用对NMT-1 R细胞有协同作用。但对放射敏感的NMT-1细胞无增强作用。两种细胞株的凋亡发生率、细胞周期分布和DNA修复能力在单独的碳束照射和碳束照射联合足叶乙甙或顺铂的作用下均无变化。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Interaction of high-LET heavy-ion irradiation and chemotherapeutic agents on two cell lines with different radiosensitivities and different p53 status in vitro.

高 LET 重离子照射和化疗药物对体外具有不同放射敏感性和不同 p53 状态的两种细胞系的相互作用。

DOI：
发表时间：
2006
期刊：
Radiotherapy ＆ Oncology 81
影响因子：
0
作者：
Osada H;Honda N;Takahashi T;Oku S;Abe A;Watanabe W;Okada T;Ohno H;Hondo M;Nishimura K;Takahashi T;Takahashi T;Takahashi T
通讯作者：
Takahashi T

MRI Perfusion Study in Head And Neck Cancers for Early Prediction of Response to Radiotherapy: A Preliminary Study.

头颈癌 MRI 灌注研究用于早期预测放射治疗的反应：一项初步研究。

DOI：
发表时间：
2006
期刊：
Austral-Asian Journal of Cancer 5
影响因子：
0
作者：
Osada H;Honda N;Takahashi T;Oku S;Abe A;Watanabe W;Okada T;Ohno H;Hondo M;Nishimura K;Takahashi T;Takahashi T
通讯作者：
Takahashi T

Aggressive multiple lung metastases from intracranial atypical meningioma

颅内非典型脑膜瘤侵袭性多发性肺转移