Interaction of heavy-ion microbeam and chemotherapeutic agents in vitro

重离子微束与体外化疗药物的相互作用

• 批准号: 20591504
• 负责人: TAKAHASHI Takeo
• 金额: $ 2.41万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591504/
• 关键词: 重粒子線; バイスタンダー効果; p53; 抗癌剤; DNA修復; 抗がん剤; マイクロビーム; 増感効果; Ku70

It is known that relative biological effectiveness (RBE) of high-LET heavy-ion irradiation is higher than that of low-LET radiation such as X-ray. Monotherapy of charged particles has been applied for several tumors. We investigate the differences between two tumor cell lines with different radiosensitivities in sensitivity to high-LET heavy ion beam and in sensitizing effect and the mechanism of cisplatin and etoposide in combination with heavy-ion beam. Etoposide showed a synergistic effect in combination with carbon beam irradiation in radioresistant NMT-1R cells. However, there was no enhancement effect in radiosensitive NMT-1 cells. On the other hand, cisplatin had no enhancement in both cell lines. There was no differences in the pattern of G2 arrest among Carbon-beam + etoposide, Carbon-beam + cisplatin, and Carbon-beam alone 24 hours after irradiation. The increase in amount of apoptosis and the inhibition of Ku70 protein expression were found in the combination of carbon beam irradiation and etoposide in NMT-1 cells. There were bystander effects after heavy-ion microbeam irradiation.

众所周知，高LET重离子辐射的相对生物效应(RBE)高于X射线等低LET辐射。带电粒子的单一疗法已经应用于几种肿瘤。我们研究了两种不同放射敏感性的肿瘤细胞系对高LET重离子束的敏感性和增敏作用的差异，以及顺铂和依托泊苷联合重离子束的作用机制。依托泊苷联合碳束照射对耐受辐射的NMT-1R细胞有协同作用。但对辐射敏感的NMT-1细胞无增强作用。另一方面，顺铂对两种细胞株均无促进作用。照射后24小时，碳束+依托泊苷、碳束+顺铂、碳束单独照射对细胞G2期的抑制作用无明显差异。碳束照射与依托泊苷联合作用可增加NMT-1细胞的凋亡率，抑制Ku70蛋白的表达。重离子微束照射后出现旁观者效应。

项目成果

High-dose-rate intracavity brachytherapy combined with external beam radiotherapy for stage III B adenocarcinoma of the uterine cervix in japan : A multi-institutional study of Japanene society of therapeutic radiorogy and oncology 2006-2007.

日本高剂量腔内近距离放射治疗联合体外放射治疗治疗 III B 期宫颈腺癌：日本治疗放射学和肿瘤学会 2006-2007 年多机构研究。

• 发表时间: 2010
• 期刊: Jpn J Clini Oncol 8
• 作者: Niibe Y;Kenjo M;Onishi H;Ogawa Y;Kazumoto T;Ogino I;Tsujino K;Harima Y;Takahashi T;Anbai A;Tsuchiba E;Toita T;Takemoto M;Yamashita H;Hayakawa K
• 通讯作者: Hayakawa K

Preclinical biological assessment for treatment planning of carbon-ion therapy.

碳离子疗法治疗计划的临床前生物学评估。

• 发表时间: 2010
• 作者: Ishikawa H;Nonaka T;Sakurai H;Tamaki Y;Kitamoto Y;Ebara T;Shioya M;Noda S;Shirai K;Suzuki Y;Takahashi T. Nakano T;Ishikawa H;Takahashi T;Takahashi T;Takahashi T;Yoshida Y
• 通讯作者: Yoshida Y

mechanism of enhancement effect of carbon-beam irradiation and topoisomerase ll inhibitor on two cell lines with different radiosensitivities and different p53 status in vitro.

碳束照射和拓扑异构酶II抑制剂对不同放射敏感性和不同p53状态的两种细胞系的体外增强作用机制。

• 发表时间: 2009
• 期刊: 37th Annual Meeting of the European Radiation research Society (Praque August 26-29)
• 作者: Takahashi T;Yoshida Y;Musya A;Kawamura H;Ando K;Nakano T
• 通讯作者: Nakano T

Metastatic angiosarcoma of the lung with alveolar hemorrhage

• DOI: 10.1007/s11604-009-0352-1
• 发表时间: 2009-11-01
• 期刊: JAPANESE JOURNAL OF RADIOLOGY
• 影响因子: 2.1
• 作者: Saitoh, Jun-ichi;Sakurai, Hideyuki;Nakano, Takashi
• 通讯作者: Nakano, Takashi

Intracavitary Combined with CT-guided Interstitial Brachytherapy for Locally Advanced Uterine Cervical Cancer: Introduction of the Technique and a Case Presentation

• DOI: 10.1269/jrr.10091
• 发表时间: 2011-01-01
• 期刊: JOURNAL OF RADIATION RESEARCH
• 影响因子: 2
• 作者: Wakatsuki, Masaru;Ohno, Tatsuya;Nakano, Takashi
• 通讯作者: Nakano, Takashi