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Study on bacterial ecology and structural permeability within dental plaque using a quantitative method for mapping the distribution pattern of plaque bacteria

使用定量方法研究牙菌斑内的细菌生态和结构渗透性以绘制牙菌斑细菌的分布模式

基本信息

批准号：
17592192
负责人：
KATO Kazuo
金额：
$ 2.41万
依托单位：
Aichi Gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

(1) The antimicrobial effect of SnF_2 gel treatment on dental plaque in vivo was evaluated, using a depth-specific analysis of Streptococcus mutans from the outermost to the innermost plaque. The patterns of positive fractions for S. mutans were highly varied among the subjects. Although frequencies of plaque layer containing S. mutans were higher after the treatment, indicating that the treatment made their habitat in plaque significantly wider, volume of plaque layers were significant lower after the treatment. The results suggested that the application of SnF_2 gel might indeed increase the porosity of plaque, creating a larger habitat for cariogenic bacteria particularly in deeper regions.(2) Consuming both yogurt products containing L. reuteri with sucrose and without either sweetener significantly reduced the detection rates of S. mutans in saliva, although the product including xylitol instead of sucrose couldn't produce significant results. The consumption of any yogurt product … More s could not be linked to any significant reductions of S. sobrinus. However, placebo-controlled trial demonstrated that consuming yogurt with L. reuteri could not significantly reduce the habitat for mutans streptococci within plaque in vivo.(3) Species-specific nested, two-step PCR amplification based upon 16S ribosomal RNA genes was employed to develop the rapid and sensitive detection method for cariogenic bacteria in human plaque. A set of PCR primers universal for bacterial 16S ribosomal RNA gene (for the first PCR), and two sets of PCR primers specific for either S. mutans or S. sobrinus designed from the alignment of the 16S rRNA gene sequences (for the second PCR) were used. Plaque samples from 8 volunteers were used for the nested PCR, and the nested PCR was shown to be more sensitive rather than a direct PCR using specific primer sets.(4)Observations of microradiography revealed great differences in acid resistance between NaF and MFP. NaF treated enamel showed acid resistant at only superficial area. However MFP treated enamel has thick and deep acid resistant layer. Therefore, NaF could not penetrate into the enamel while MFP penetrated deeper. The results showed that NaF and MFP made an interesting contrast at investment of acid resistance on acidic condition. Less

(1)通过对变形链球菌从牙菌斑最外层到最内层进行深度特异性分析，评估了SnF_2凝胶处理对牙菌斑的体内抗菌效果。受试者中变形链球菌阳性分数的模式差异很大。虽然处理后含有变形链球菌的菌斑层频率较高，表明处理后其在菌斑中的栖息地显着扩大，但处理后菌斑层的体积显着降低。结果表明，SnF_2凝胶的应用确实可能增加牙菌斑的孔隙率，为致龋菌创造更大的栖息地，特别是在较深的区域。(2)同时食用含罗伊氏乳杆菌和蔗糖的酸奶产品和不含任何甜味剂的酸奶产品均显着降低了唾液中变形链球菌的检出率，尽管用木糖醇代替蔗糖的产品不能产生显着的结果。任何酸奶产品的消费都与 S. sobrinus 的显着减少无关。然而，安慰剂对照试验表明，食用含有罗伊氏乳杆菌的酸奶并不能显着减少体内斑块内变形链球菌的栖息地。(3)采用基于16S核糖体RNA基因的物种特异性巢式两步PCR扩增技术，开发了快速、灵敏的人体斑块中致龋细菌的检测方法。使用一组对细菌 16S 核糖体 RNA 基因通用的 PCR 引物（用于第一次 PCR），以及两组根据 16S rRNA 基因序列比对设计的对变形链球菌或远缘链球菌具有特异性的 PCR 引物（用于第二次 PCR）。使用8名志愿者的噬菌斑样本进行巢式PCR，结果显示巢式PCR比使用特定引物组的直接PCR更敏感。(4)显微放射照相观察显示NaF和MFP的耐酸性存在很大差异。 NaF 处理的牙釉质仅在表面区域表现出耐酸性。然而，经MFP处理的牙釉质具有又厚又深的耐酸层。因此，NaF无法渗透到牙釉质中，而MFP则渗透得更深。结果表明，NaF和MFP在酸性条件下的耐酸性投资方面形成了有趣的对比。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

950ppmFフッ化ナトリウムおよびモノフルオロリン酸ナトリウムのエナメル質耐酸性に及ぼす影響

950ppmF氟化钠和单氟磷酸钠对搪瓷耐酸性的影响

DOI：
发表时间：
2007
期刊：
口腔衛生学会雑誌 57
影响因子：
0
作者：
Manaho Yamagishi;Toshio Kobayashi;Yuko Nakamura;Yamagishi Manaho;山岸まなほ;Manaho Yamagishi;山岸まなほ;Manaho Yamagishi;小林敏生;小林敏生;小林敏生;山岸敦
通讯作者：
山岸敦

Nested PCR for the sensitive detection of cariogenic bacteria.

用于灵敏检测致龋菌的巢式 PCR。

DOI：
发表时间：
2007
期刊：
Cariology Today 3 & 4
影响因子：
0
作者：
Takuichi;Sato;Renya Sato;Takuichi Sato
通讯作者：
Takuichi Sato

Influence of yogurt products containing Lactobacillus reuteri on distributions of mutans streptococci within dental plaque.

含有罗伊氏乳杆菌的酸奶产品对牙菌斑内变形链球菌分布的影响。

DOI：
发表时间：
2007
期刊：
Interface Oral Health Science 2007
影响因子：
0
作者：
Sato;T.;Abiko Y;Sato T;Sato R;Ito Y;Masaki M;Matsuyama J;Kato K
通讯作者：
Kato K

Distribution profiles of Streptococcus mutans within dental plaque formed in vivo under xylitol rinsing regimen

木糖醇冲洗方案下体内形成的牙菌斑内变形链球菌的分布概况

DOI：
发表时间：
2007
期刊：
Cariology Today Vol.3-4
影响因子：
0
作者：
Manaho Yamagishi;Toshio Kobayashi;Yuko Nakamura;Yamagishi Manaho;山岸まなほ;Manaho Yamagishi;山岸まなほ;Manaho Yamagishi;小林敏生;小林敏生;小林敏生;山岸敦;Keiko FUKUI
通讯作者：
Keiko FUKUI

ヨーグルト中のLactobacillus reuteriによるう蝕関連菌抑制効果におよぼす糖質の影響に関する研究

酸奶中碳水化合物对罗伊氏乳杆菌抑制龋相关菌影响的研究