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Study on bacterial ecology and structural permeability within dental plaque using a quantitative method for mapping the distribution pattern of plaque bacteria

使用定量方法研究牙菌斑内的细菌生态和结构渗透性以绘制牙菌斑细菌的分布模式

基本信息

批准号：
17592192
负责人：
KATO Kazuo
金额：
$ 2.41万
依托单位：
Aichi Gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

(1) The antimicrobial effect of SnF_2 gel treatment on dental plaque in vivo was evaluated, using a depth-specific analysis of Streptococcus mutans from the outermost to the innermost plaque. The patterns of positive fractions for S. mutans were highly varied among the subjects. Although frequencies of plaque layer containing S. mutans were higher after the treatment, indicating that the treatment made their habitat in plaque significantly wider, volume of plaque layers were significant lower after the treatment. The results suggested that the application of SnF_2 gel might indeed increase the porosity of plaque, creating a larger habitat for cariogenic bacteria particularly in deeper regions.(2) Consuming both yogurt products containing L. reuteri with sucrose and without either sweetener significantly reduced the detection rates of S. mutans in saliva, although the product including xylitol instead of sucrose couldn't produce significant results. The consumption of any yogurt product … More s could not be linked to any significant reductions of S. sobrinus. However, placebo-controlled trial demonstrated that consuming yogurt with L. reuteri could not significantly reduce the habitat for mutans streptococci within plaque in vivo.(3) Species-specific nested, two-step PCR amplification based upon 16S ribosomal RNA genes was employed to develop the rapid and sensitive detection method for cariogenic bacteria in human plaque. A set of PCR primers universal for bacterial 16S ribosomal RNA gene (for the first PCR), and two sets of PCR primers specific for either S. mutans or S. sobrinus designed from the alignment of the 16S rRNA gene sequences (for the second PCR) were used. Plaque samples from 8 volunteers were used for the nested PCR, and the nested PCR was shown to be more sensitive rather than a direct PCR using specific primer sets.(4)Observations of microradiography revealed great differences in acid resistance between NaF and MFP. NaF treated enamel showed acid resistant at only superficial area. However MFP treated enamel has thick and deep acid resistant layer. Therefore, NaF could not penetrate into the enamel while MFP penetrated deeper. The results showed that NaF and MFP made an interesting contrast at investment of acid resistance on acidic condition. Less

(1)采用从菌斑最外层到最内层的变形链球菌深度特异性分析法，评价了SnF_2凝胶处理对牙菌斑的体内抗菌效果。研究了S.变异体在受试者中差异很大。虽然含S.处理后变链菌的数量明显增加，表明处理后变链菌在菌斑中的栖息地明显变宽，菌斑层体积明显降低。结果表明，应用SnF_2凝胶确实可能增加牙菌斑的孔隙度，为致龋细菌创造更大的栖息地，特别是在较深的区域。(2)食用这两种酸奶产品含有L。reuteri与蔗糖和没有任何甜味剂显着降低检出率S。唾液中的变形杆菌，虽然包括木糖醇而不是蔗糖的产品不能产生显著的结果。任何酸奶产品的消费 ...更多信息 s不能与S的任何显著降低相联系。sobrinus。然而，安慰剂对照试验表明，食用酸奶与L。reuteri不能显著减少体内菌斑内变形链球菌的栖息地。(3)建立了一种基于16S核糖体RNA基因的种特异性巢式两步法PCR检测人牙菌斑致龋菌的方法。一套通用于细菌16S核糖体RNA基因的PCR引物（用于第一次PCR）和两套特异于S.变形链球菌或S.使用从16S rRNA基因序列的比对设计的sobrinus（用于第二PCR）。来自8名志愿者的斑块样品用于巢式PCR，并且巢式PCR显示出比使用特异性引物组的直接PCR更敏感。（4）显微放射照相观察表明，NaF和MFP的耐酸性存在很大差异。NaF处理的釉质仅在表浅区域显示抗酸性。但MFP处理后的釉质具有较厚较深的耐酸层。因此，NaF不能渗透到釉质中，而MFP渗透得更深。结果表明，在酸性条件下，NaF和MFP的耐酸性表现出明显的差异。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

950ppmFフッ化ナトリウムおよびモノフルオロリン酸ナトリウムのエナメル質耐酸性に及ぼす影響

950ppmF氟化钠和单氟磷酸钠对搪瓷耐酸性的影响

DOI：
发表时间：
2007
期刊：
口腔衛生学会雑誌 57
影响因子：
0
作者：
Manaho Yamagishi;Toshio Kobayashi;Yuko Nakamura;Yamagishi Manaho;山岸まなほ;Manaho Yamagishi;山岸まなほ;Manaho Yamagishi;小林敏生;小林敏生;小林敏生;山岸敦
通讯作者：
山岸敦

Nested PCR for the sensitive detection of cariogenic bacteria.

用于灵敏检测致龋菌的巢式 PCR。

DOI：
发表时间：
2007
期刊：
Cariology Today 3 & 4
影响因子：
0
作者：
Takuichi;Sato;Renya Sato;Takuichi Sato
通讯作者：
Takuichi Sato

Influence of yogurt products containing Lactobacillus reuteri on distributions of mutans streptococci within dental plaque.

含有罗伊氏乳杆菌的酸奶产品对牙菌斑内变形链球菌分布的影响。

DOI：
发表时间：
2007
期刊：
Interface Oral Health Science 2007
影响因子：
0
作者：
Sato;T.;Abiko Y;Sato T;Sato R;Ito Y;Masaki M;Matsuyama J;Kato K
通讯作者：
Kato K

Distribution profiles of Streptococcus mutans within dental plaque formed in vivo under xylitol rinsing regimen

木糖醇冲洗方案下体内形成的牙菌斑内变形链球菌的分布概况

DOI：
发表时间：
2007
期刊：
Cariology Today Vol.3-4
影响因子：
0
作者：
Manaho Yamagishi;Toshio Kobayashi;Yuko Nakamura;Yamagishi Manaho;山岸まなほ;Manaho Yamagishi;山岸まなほ;Manaho Yamagishi;小林敏生;小林敏生;小林敏生;山岸敦;Keiko FUKUI
通讯作者：
Keiko FUKUI

ヨーグルト中のLactobacillus reuteriによるう蝕関連菌抑制効果におよぼす糖質の影響に関する研究

酸奶中碳水化合物对罗伊氏乳杆菌抑制龋相关菌影响的研究