THE MINERAL DISTRIBUTIONS AND THEIR PROMOTION OF FLUORIDE RETENTION IN TH EPLAQUE in vivo EXPOSED TO A MINERAL ENRICHING MOUTHRINSE

本研究的目的是澄清矿物质（钙和磷）的分布和促进体内暴露于富含矿物质的漱口水的牙菌斑中的氟保留，然后估计因素，这对促进牙菌斑中的氟保留有影响。11名同意的受试者（20-31岁）在上磨牙的颊面上使用含有菌斑容器（直径2 mm）的采样装置。牙菌斑形成3天后，指导受试者用5种冲洗液中的一种冲洗，每天3次，连续4天。每种溶液都是通过从富含矿物质的漱口液（尿素500 mM，CaCl_2 20 mM，Na_2HPO_4 12 mM，Na_2PO_3F（MFP）5 mM，pH_5）中排除一些成分而制成的。使用不同的溶液间隔地重复噬菌斑收集。暴露于不含任何成分的安慰剂溶液的菌斑样品作为对照。最后一次冲洗后24小时，将样品冷冻干燥，然后包埋在甲基丙烯酸酯混合物中。使用超薄切片机，从牙菌斑外表面向牙釉质重复切割一系列平行于牙齿表面的牙菌斑切片（6 μ m处4个，然后3 μ m处2个）。较薄的切片用于面积测量，以评价样品体积。将较厚的切片收集成几个一致的标本。牙菌斑中的氟化物和矿物质分布通过Kato等Archs oral Biol.42：521-525，1997报道的方法进行分析。氟潴留增加，但在更深的斑块层。暴露于含有MEP和矿物质的溶液的样本中，氟化物浓度（0.5-3.6nmol/mm^3）显着升高，在全口含漱液中这种趋势更大。钙和磷水平显着增加，只有在牙菌斑暴露于矿物质丰富的漱口水。然而，方差分析表明，受试者之间的差异将是一个更强大的因素比类型的漱口水氟保留在菌斑。

The aim of this study was to clarify the mineral (Ca and P) distributions and their promotion of fluoride retention in the plaque in vivo exposed to a mineral enriching mouthrinse and then to estimate factors, which have an influence on the promotion of fluoride retention in the plaque. 11 subjects consenting (20-31 years), had sampling devices containing plaque receptacles (2mm in diameter) to buccal surfaces of upper molars. After plaque had formed over 3 days, subjects were instructed to rinse with one of five rinsing solutions three times a day on 4 consecutive days. Each solution was made by excluding some ingredients out of a mineral enriching mouthrinse (urea 500mM, CaCl_2 20mM, Na_2HPO_4 12mM, Na_2PO_3F(MFP) 5mM, pH_5). Plaque collections were repeated at intervals using different solutions. Plaque samples exposed to a placebo solution with no ingredients served as control. 24 hours after the last rinsing, the sample was freeze-dried and then embedded in a methacrylate mixture. A series of plaque sections parallel with the tooth surface (4 at 6mum then 2 at 3mum) were repeatedly cut from the outer plaque surface towards the enamel, using an ultramicrotome. The thinner sections were used for area measurement to evaluate sample volume. The thicker sections were collected into several congruous specimens. Fluorides and mineral distributions in plaque were analyzed by a method reported by Kato et al. Archs oral Biol. 42 : 521-525, 1997. Fluoride retention increased except in the deeper plaque layers. Fluoride concentrations (0.5-3.6nmol/mm^3) significantly rose in the samples exposed to solutions containing both MEP and minerals, this tendency being greater in the full mouthrinse. Both calcium and phosphorus levels significantly increased only in the plaque exposed to the mineral enriching mouthrinse. However, ANOVA indicated a difference between subjects would be a stronger factor than the type of mouthrinse for fluoride retention in plaque.