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The interaction between vasopressin and oxytocin neurons after central salt loading

中枢盐负荷后加压素和催产素神经元之间的相互作用

基本信息

批准号：
13670068
负责人：
KATO Kazuo
金额：
$ 1.86万
依托单位：
Miyazaki Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

The interaction between vasopressin and oxytocin neurons.We examined the effect of central salt loading on the neuronal activity in both vasopressin (AVP) and oxytocin (OT) neurons using double labeling techniques of immunohistochemistry. After blocking the endogenous vasopressin with vasopressin V_1 receptors antagonist, the neuronal activity ratio, which was calculated by divided the number of Fos positive neurons with the number of AVP or OT neurons, increased in the paraventricular nucleus (PVN) of the hypothalamus. On the other hand, the ratio in the supraoptic nucleus (SON) was conversely decreased. This decreased neuronal activity in the SON was compatible with the decreased systemic OT levels. Endogenous vasopressin induced by central salt loading might facilitate the secretion of OT to the peripheral circulation.The role of endogenous vasopressin was modulated in the heart failure models.We made the congestive heart failure (CHF) model rats by ligating the left coronary artery … More and used them after more than three weeks recovery. Then, hypertonic saline (HS : 0.67 M) were administered centrally to two groups. One is pretreated with vasopressin V_1 receptors antagonist and the other is administered with HS only. We divided the PVN into four regions for evaluation, medial (PVM), lateral (PVL), dorsomedial cap (PVDC), and entire PVN (e-PVN). After blocking the endogenous vasopressin by vasopressin V_1 receptors antagonist, the neuronal activity in the PVDC was enhanced in the normal rats group, but conversely the facilitation was disappeared in the CHF group compared with each HS-administered alone group, respectively.By what range does the pressor response during central salt loading become stress?Previously, we showed that administration of high concentrated-HS (1.0 M) leveled off the neuronal activity in the PVN and SON. Then, we hypothesized that the greater pressor response might suppress the neuronal activity as previously reported. Although we tried to counter the pressor response during 1.0 M HS administration using sodium nitroprusside (SNP) from 26.5 ± 4.0 mmHg to -3.4 ± 3.8 mmHg, the neuronal activity in the PVN and SON changed more suppressed. It is suggested that to manipulate the blood pressure forcibly within normal range might become kind of stressor. Less

加压素（AVP）和催产素（OT）神经元之间的相互作用：采用免疫组织化学双标记技术，研究了中枢盐负荷对AVP和OT神经元活动的影响。用加压素V_1受体拮抗剂阻断内源性加压素后，下丘脑室旁核（PVN）内Fos阳性神经元数除以AVP或OT神经元数计算的神经元活动比率增加。而视上核（SON）的比值则相反。SON中神经元活性的降低与全身OT水平的降低相一致。中枢盐负荷诱导的内源性血管加压素可促进OT向外周循环的分泌，内源性血管加压素在心力衰竭模型中的作用受到调节 ...更多信息并在恢复三周多后使用。然后，高渗盐水（HS：0.67 M）集中管理到两组。一组用加压素V_1受体拮抗剂预处理，另一组仅用HS处理。我们将PVN分为四个区域进行评价，内侧（PVM）、外侧（PVL）、背内侧帽（PVDC）和整个PVN（e-PVN）。用加压素V_1受体拮抗剂阻断内源性加压素后，正常组PVDC神经元活动增强，而CHF组PVDC神经元活动的易化作用与HS单独给药组比较均消失。以前，我们表明，管理高浓度的HS（1.0 M）的PVN和SON的神经元活动趋于平稳。然后，我们假设更大的升压反应可能抑制神经元的活动，如以前报道的。在1.0 M HS给药期间，虽然我们试图用硝普钠（SNP）从26.5 ± 4.0 mmHg至-3.4 ± 3.8 mmHg对抗升压反应，但PVN和SON的神经元活动变化更受抑制。结果提示，强迫性血压控制在正常范围内可能成为一种应激源。少