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Elucidation of origin of citrus based on information from chromosome analysis

根据染色体分析信息阐明柑橘的起源

基本信息

批准号：
18580028
负责人：
YAMAMOTO Masashi
金额：
$ 2.27万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580028/
关键词：
horticulture genetic resources evolution citrus Aurantioideae chromosome FISH CMA 育種

项目摘要

Although citrus, belong to Aurantioideae which is one of the seven subfamilies of Rutaceae, is the one of the most important fruit crop in the world, its origin and phylogenic relationships among species in Aurantioideae has been unclarified. Therefore in this study, we conducted chromosome analysis to elucidate the phylogenic relationships and evolution within Aurantioideae.The chromosome number analysis revealed that almost all Aurantioideae species had diploid (2n=2x=18) chromosomes. Only two species were tetraploid (2n=4x=36). Glycosmis pentaphylla was a mixoploid having diploid and hexaploid (2n=6x=54) cells.Fluorochrome staining with chromomycin A3 (CMA) was used to characterize and compare the CMA banding patterns of chromosomes of 21 species from 16 genera of Aurantioideae. These chromosomes were classified into six types based on the number and position of CMA-positive bands ; A : two telomeric and one proximal band, B : one telomeric and one proximal band, C : two telomeric b … More ands, D : one telomeric band, E : without bands, and Dst : type D with a satellite chromosome. Each species possessed one to four types of chromosomes and unique CMA banding patterns. The advanced genera in each tribe and subtribe such as Merrillia and Feroniella showed heterochromatin (CMA-positive bands). rich chromosome configuration in general. Swinglea glutinosa without heterochromatin rich chromosome, which is considered to be the most primitive chromosome configuration, seems to be the key genus for clarifying the evolution of the tribe Citreae.Fluorescent in situ hybridization (FISH) using ribosomal RNA genes (rDNA) is a powerful tool for phylogenic studies. Thus FISH of 5S rDNA was carried out in seven genera from primitive to advanced one. Two 5S rDNA sites were detected in six out of seven genera. Poncirus had six 5S rDNA sites. From these results, two seem to be a base number of 5S rDNA sites in Aurantioideae. Diversity of number of 5S rDNA sites occurred after the true citrus fruit trees were differentiated. Less

柑桔属芸香科（Rutaceae）七亚科之一的枳壳亚科（Aurantioideae），是世界上最重要的水果作物之一，但其起源及各亚科种间的亲缘关系一直不清楚。染色体数目分析表明，几乎所有的枳壳亚科植物都具有二倍体（2n=2x=18）染色体。只有两个种是四倍体（2n=4x=36）。五叶小橘（Glycosmispentaphylla）是一种二倍体和六倍体（2n=6x=54）的混倍体，采用色霉素A3（CMA）荧光染色法，对枳壳亚科16属21种植物的染色体CMA带型进行了研究和比较。根据CMA阳性带的数目和位置将这些染色体分为六种类型：A：2条端粒带和1条近端带，B：1条端粒带和1条近端带，C：2条端粒带B ...更多信息 D型：一条端粒带，E型：无端粒带，Dst型：有随体染色体。每个种都具有1 ~ 4种染色体类型和独特的CMA带型。各族和亚族中的高级属如Merrillia和Feroniella均显示异染色质（CMA阳性带）。染色体构型丰富。Swinglea sporsosa是一个不含异染色质的染色体组，被认为是最原始的染色体构型，是研究Citrus族进化的关键属，利用核糖体RNA基因（rDNA）进行荧光原位杂交（FISH）是研究Citrus族染色体组发生的有力工具。因此，对从原始到高级的7个属进行了5SrDNA的FISH分析。在7个属中的6个属中检测到2个5SrDNA位点。枳属有6个5SrDNA位点。从这些结果来看，2可能是枳壳亚科的5SrDNA位点的一个碱基数。5SrDNA位点数的多样性发生在真柑橘树分化后。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

ミカン亜科植:物における染色体のCMA染色

芸香科：植物染色体的 CMA 染色

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Yamamoto;M;山本雅史;山本雅史;山本雅史・A.Asadi Abkenar・松本亮司・久保達也・冨永茂人
通讯作者：
山本雅史・A.Asadi Abkenar・松本亮司・久保達也・冨永茂人

Application of fluorescent staining of chromosomes to genetic studies in citcus

染色体荧光染色在柑橘遗传研究中的应用

DOI：
发表时间：
2007
期刊：
Japanese Journal of Plant Science 1
影响因子：
0
作者：
Yamamoto;M.
通讯作者：
M.

ミカン亜科植物における染色体のCMA染色

芸香科植物染色体的 CMA 染色

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Yamamoto;M.;A. Asadi;Abkenar;R. Matsumoto;T. Kubo;S. Tominaga;山本雅史・A. Asadi Abkenar・松本亮司・久保達也・冨永茂人
通讯作者：
山本雅史・A. Asadi Abkenar・松本亮司・久保達也・冨永茂人

Application of Fluorescent Staining of Chromosomes to Genetic Studies in Citrus