Investigation for the mechanisms of metastasis and growth of HCC via chemokine receptors

我们采用实时荧光定量PCR技术研究趋化因子受体在肝癌细胞系中的表达。CXC趋化因子受体cxcr4在PLC/PRF/5细胞上强表达，CC趋化因子受体CCR6在HuH7、Al和HepG2细胞上强表达。CXCR4配体sdf -1由PLC/PRF/5细胞分泌，CCR6配体MIP-3 α由HuH7、Al和HepG2细胞分泌。在体外培养HuH7细胞的培养基中加入MIP-3 α后，HuH7细胞的存活率增加。根据这些结果，我们推测一定数量的HCC细胞的生长是由于肝癌细胞和淋巴细胞在发炎的人肝脏中分泌的趋化因子增加。趋化因子和趋化因子受体系统可能通过旁分泌或自分泌的方式参与HCC的生长机制之一，我们也使用表达CXCR4的Al细胞系进行了实验，但我们没有发现该系统在这种情况下确实起作用。

We investigated the expression of chemokine receptors on HCC cell lines by real time PCR. CXC chemokine receoptor CXCR 4 was strongly expressed on PLC/PRF/5 cells, and CC chemokine receptor CCR6 was strongly expressed on HuH7, Al, and HepG2 cell lines.The ligand of CXCR4, SDF-1was secreted by PLC/PRF/5 cells, and the ligand of CCR6, MIP-3 alpha was secreted by HuH7, Al, and HepG2 cell lines.Addition of MIP-3 alpha to the medium of HuH7 cells increased the number of viable HuH7 cells in vitro. From these results, we speculate that the growth of a certain number of HCC cells was attributable to the increased chemokines secreted by HCC cells and lymphocytes in the inflamed lhuman liver. Chemokine and chenokine receptor system may by one of the mechanisms of the growth of HCC in a paracrine or autocrine way..We also performed experiments using Al cell line which express CXCR4, but we could not find that the system was actually working on this occasion.