Expression of heparanase in the hepatic tissue of hepatitis patients and its clinical significance

慢性丙型肝炎(CH-C)患者CXC趋化因子受体3(CXCR3)阳性的外周血单个核细胞(PBMC)比例随肝炎加重而升高。在某些干扰素-α治疗的CH-C中，CXCR3阳性的PBMC比例在干扰素治疗过程中也有所增加。采用Taq Man聚合酶链式反应技术检测了6株人肝癌细胞株(HH-7、、/PRF/5、HEPG2、HEP3B、HLE、HLF)和常肝细胞株趋化因子受体和肝素酶(CCR110HXCR1~4)的表达。4种趋化因子受体CCR6、CCR11、CXCR4、CXCR11在部分肝癌细胞系中表达增强，除HepG2外，所有肝癌细胞系中乙酰肝素酶的mRNA表达均增强。

In chronic hepatitis C (CH-C) patients, the ratio of CXC chemokine receptor 3 (CXCR3) positive peripheral blood mononuclear cells (PBMC) increased during the exacerbation of the hepatitis. In some interferon (IFN)-alpha treated CH-C, the ratio of CXCR3 positive PBMC also increased during the IFN treatment. The expression of heparanase in the hepatic tissues as well as in the PBMC is under investigation.The expression of chemokine receptors and heparanase (CCR1〜10、CXCR1〜4) in 6 cell lines derived from human hepatoma(HuH-7, PLC/PRF/5, HepG2, Hep3B, HLE, HLF) and Chang liver cell were examined by Taq Man PCR. The mRNA expression of 4 chemokine receptors (CCR6, CCR11, CXCR4, CXCR11) increased in several of the hepatoma cell lines, and the mRNA expression of heparanase increased in all hepatoma cell lines except for HepG2.