Expression of heparanase in the hepatic tissue of hepatitis patients and its clinical significance

肝炎患者肝组织中乙酰肝素酶的表达及其临床意义

• 批准号: 12670509
• 负责人: ITOH Yoshito
• 金额: $ 2.11万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670509/
• 关键词: chemokine receptor; heparanase; hepatitis; hepatoma cell lines; ケモカイン

In chronic hepatitis C (CH-C) patients, the ratio of CXC chemokine receptor 3 (CXCR3) positive peripheral blood mononuclear cells (PBMC) increased during the exacerbation of the hepatitis. In some interferon (IFN)-alpha treated CH-C, the ratio of CXCR3 positive PBMC also increased during the IFN treatment. The expression of heparanase in the hepatic tissues as well as in the PBMC is under investigation.The expression of chemokine receptors and heparanase (CCR1〜10、CXCR1〜4) in 6 cell lines derived from human hepatoma(HuH-7, PLC/PRF/5, HepG2, Hep3B, HLE, HLF) and Chang liver cell were examined by Taq Man PCR. The mRNA expression of 4 chemokine receptors (CCR6, CCR11, CXCR4, CXCR11) increased in several of the hepatoma cell lines, and the mRNA expression of heparanase increased in all hepatoma cell lines except for HepG2.

在慢性丙型肝炎（CH-C）患者中，CXC趋化因子受体3 （CXCR3）阳性外周血单核细胞（PBMC）比例随着肝炎的加重而增加。在某些干扰素(IFN)- α治疗的CH-C中，CXCR3阳性PBMC的比例也在IFN治疗期间增加。肝素酶在肝组织和PBMC中的表达正在研究中。采用Taq Man PCR检测了6株人肝癌细胞系（hh -7、PLC/PRF/5、HepG2、Hep3B、HLE、HLF）和Chang肝细胞中趋化因子受体和肝素酶（CCR1 ~ 10、CXCR1 ~ 4）的表达。4种趋化因子受体（CCR6、CCR11、CXCR4、CXCR11） mRNA表达在多种肝癌细胞系中均升高，肝素酶mRNA表达在除HepG2外的所有肝癌细胞系中均升高。

项目成果

Itoh Y: "Time course profille and cell-type-specltic production of monokino induced by interferon-γ in Coucanavulin A-induccd bopatic Injury in mice"Scand J Gastroenterol. 36. 1344-1351 (2001)

Itoh Y：“在小鼠中由干扰素-γ诱导的单核素的时间进程概况和细胞类型特异性产生”Scand J Gastroenterol。 36. 1344-1351 (2001)

Itoh Y, et al.: "Clinical significance of elevated serum interferon-inducible protein-10 levels in hepatitis G virus carriers with persistently normal serum transaminase levels"J Viral Hepat. 8. 341-8 (2001)

Itoh Y 等人：“血清转氨酶水平持续正常的 G 型肝炎病毒携带者血清干扰素诱导蛋白 10 水平升高的临床意义”J Viral Hepat。

Itoh Y, et al.: "Time course profile and cell-type specific production of monokmine induced by interferon gamma (MIG) in Concanavalin A-induced hepatic injury in mice : compative study with interferon-inducible protein-10 (IP-10)"Scand J Gastroenterol. 36

Itoh Y 等人：“在伴刀豆球蛋白 A 诱导的小鼠肝损伤中，干扰素 γ (MIG) 诱导的单克明的时程概况和细胞类型特异性产生：与干扰素诱导蛋白 10 (IP-10) 的比较研究

Nishioji K: "Increase of chemokine interferon-inducible protein-10(IP-10)in the serum of patients with autoimmune liver diseases and increase of its mRNA expression in hepatocytes"Clin Exp Immunol. 123. 271-279 (2001)

Nishioji K：“自身免疫性肝病患者血清中趋化因子干扰素诱导蛋白 10 (IP-10) 的增加及其在肝细胞中 mRNA 表达的增加”Clin Exp Immunol。

Itoh Y: "Clinical significunce of elevated serum interferon-inducible protein-10 levels in heputitiS C virus carriers with persistently normal serum trunanminase levels"J Viral Hepat. 8. 341-348 (2001)

Itoh Y：“丙型肝炎病毒携带者血清干扰素诱导蛋白 10 水平升高的临床意义，血清 trunanminase 水平持续正常”J Viral Hepat。