Expression of heparanase in the hepatic tissue of hepatitis patients and its clinical significance

在慢性丙型肝炎（CH-C）患者中，CXC趋化因子受体3（CXCR 3）阳性外周血单个核细胞（PBMC）的比例随着肝炎的加重而增加。在一些干扰素（IFN）-α治疗的CH-C中，CXCR 3阳性PBMC的比率在IFN治疗期间也增加。采用TaqMan PCR方法检测了6株人肝癌细胞系（HuH-7、PLC/PRF/5、HepG 2、Hep 3B、HLE、HLF）和Chang肝细胞中趋化因子受体（CCR 1 - 10、CXCR 1 - 14）和乙酰肝素酶（heparanase）的表达。4种趋化因子受体（CCR 6、CCR 11、CXCR 4、CXCR 11）的mRNA在几种肝癌细胞系中表达增加，乙酰肝素酶的mRNA在除HepG 2外的所有肝癌细胞系中表达增加。

In chronic hepatitis C (CH-C) patients, the ratio of CXC chemokine receptor 3 (CXCR3) positive peripheral blood mononuclear cells (PBMC) increased during the exacerbation of the hepatitis. In some interferon (IFN)-alpha treated CH-C, the ratio of CXCR3 positive PBMC also increased during the IFN treatment. The expression of heparanase in the hepatic tissues as well as in the PBMC is under investigation.The expression of chemokine receptors and heparanase (CCR1〜10、CXCR1〜4) in 6 cell lines derived from human hepatoma(HuH-7, PLC/PRF/5, HepG2, Hep3B, HLE, HLF) and Chang liver cell were examined by Taq Man PCR. The mRNA expression of 4 chemokine receptors (CCR6, CCR11, CXCR4, CXCR11) increased in several of the hepatoma cell lines, and the mRNA expression of heparanase increased in all hepatoma cell lines except for HepG2.