The regulation of signalling molecules in Saccharomyces Cerevisiae by inositol polyphosphate 5-phosphatases

磷酸肌醇信号分子调节肌动蛋白细胞骨架、分泌、囊泡运输和细胞生长与死亡。我们已经确定，克隆和表征了一个家庭的信号终止酶称为肌醇多磷酸5-磷酸酶（5-磷酸酶），调节磷酸肌醇信号分子。我们已经克隆和鉴定了四种不同的5-磷酸酶在酵母酿酒酵母，并证明了缺失和过表达的研究，这些酶调节肌动蛋白细胞骨架，内吞作用和分泌。本研究的目的是调查的信号复合物的5-磷酸酶形式与特定的肌动蛋白结合和/或调节蛋白，调查磷脂酰肌醇脂质磷酸酶的复杂的相互作用和它们在调节分泌的作用，从内质网和最后表征一个新的5-磷酸酶，我们最近发现。总的来说，这些研究的结果将提供新的信息functionallly重要的信号转导途径调节这一重要的酶家族。

Phosphoinositide signalling molecules regulate the actin cytoskeleton, secretion, vesicular trafficking and cell growth and death. We have identified, cloned and characterised a family of signal terminating enzymes called inositol polyphosphate 5-phosphatases (5-phosphatases) that regulate phosphoinositide signalling molecules. We have cloned and characterised four distinct 5-phosphatases in the yeast Saccharomyces Cerevisiae and demonstrated by both deletion and overexpression studies that these enzymes regulate the actin cytoskeleton, endocytosis and secretion. This research proposal aims to investigate the signalling complexes the 5-phosphatases form with specific actin binding and or regulatory proteins, investigate the complex interactions of phosphoinositide lipid phosphatases and the roles they play in regulating secretion from the endoplasmic reticulum and finally characterize a novel 5-phosphatase that we have recently identified. Collectively the outcome of these studies will provide novel information about the functionallly significant signalling pathways regulated by this important enzyme family.