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Molecular dissection of the flowering process from induction to morphogenesis in Arabidopsis

拟南芥开花过程从诱导到形态发生的分子解剖

基本信息

批准号：
18370018
负责人：
ARAKI Takashi
金额：
$ 9.82万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18370018/
关键词：
Higher plants Flowering Photoperiodism Protein mRNA Signal transduction Long-distance signal Arabidopsis シグナル伝達

项目摘要

Molecular events in FD-dependent and FD-independent flowering pathways and long-distance action of FT gene products (mRNA, protein) have been investigated. As components of the FD-dependent pathway, several Ca-dependent protein kinases (CDPKs) which may phosphorylate a C-terminal CDPK site in FD in the shoot apex were identified as FD-interactors by yeast two-hybrid assays. Biochemical demonstration of phosphorylation of FD will be the important next step in the future research. Genetic interaction between fd mutation and other flowering-related mutations was analyzed in detail and papers are currently being prepared. As an important component of the FD-independent flowering pathway, SOC1 was identified as an immediate downstream target of FT in the shoot apex. Several TCP transcription factors were identified as FT-interactors by yeast two-hybrid assays and bi-molecular fluorescent complementation assays. The role of these TCPs in flowering is currently being investigated. The long-distance action of FT in promotion of flowering was demonstrated using a local transient induction system for FT and two-shoot Y-grafting of young seedlings. The transport of FT-T7 protein from a single leaf blade to the shot apex or from a donor scion to the shoot apex of a recipient stock plants within 24-48 hours was observed. By using a synthetic FT gene with extensive synonymous substitution of all the codons in a single ORF, it was clearly shown that the mRNA sequence/structure per se is not of vital importance for the long-distance action of FT. These results have led to a conclusion that the FT protein, not mRNA, is the component of florigen.

已研究了依赖于FD和不依赖于FD的开花途径中的分子事件以及FT基因产物（mRNA、蛋白质）的远距离作用。作为FD依赖性途径的组成部分，几种钙依赖性蛋白激酶（CDPKs），可能磷酸化的C-末端CDPK网站FD在茎尖被确定为FD相互作用的酵母双杂交试验。FD磷酸化的生物学验证将是今后研究的重要一步。详细分析了fd突变与其他开花相关突变的遗传互作，目前正在准备论文。SOC 1作为FD非依赖性开花途径的重要组成部分，被确定为茎尖FT的直接下游靶点。通过酵母双杂交试验和双分子荧光互补试验，确定了几种TCP转录因子为FT相互作用因子。目前正在研究这些TCP在开花中的作用。FT促进开花的远距离作用是通过FT和幼苗双枝Y形嫁接的局部瞬时诱导系统来证明的。观察到FT-T7蛋白在24-48小时内从单个叶片运输到茎尖或从供体接穗运输到受体砧木植株的茎尖。通过使用在单个ORF中具有所有密码子的广泛同义取代的合成FT基因，清楚地表明mRNA序列/结构本身对于FT的长距离作用并不至关重要。这些结果导致了这样的结论，即FT蛋白，而不是mRNA，是成花素的组分。