Analysis of mechanisms fix human embryo implantation by crosstalk between mother and embryo through endocrine and immune system network

通过内分泌和免疫系统网络分析母体与胚胎之间的串扰固定人类胚胎着床的机制

• 批准号: 18390447
• 负责人: FUJIWARA Hiroshi
• 金额: $ 11.04万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390447/
• 关键词: implantation; embryo; endometrium; corpus luteum; pregnancv

After invading endometrium, human embryo is surrounded by maternal blood stream. Human chorionic gonadotropin (HCG) that is secreted from trophoblast of the embryo is transmitted to the ovary through blood circulation, stimulating corpus luteum function to produce progesterone which in turn maintains embryo implantation in the uterus (Crosstalk between embryo and mother by endocrine system). In these processes, maternal peripheral blood mononuclear cells (PBMC) directly contact with trophectoderm of the embryo. Therefore, we proposed that these PBMC can transmit information about the presence of the embryo to the ovary and endometrium and contribute to inducing and maintaining embryo implantation (Crosstalk between embryo and mother by immune system). Based on the above novel concept, this project successfully developed a new clinical therapy to treat infertile patients with repeated implantation failure using autologous PBMC. In this project, maternal platelets were also shown to play an important role in angiogenetic reconstruction during corpus luteum formation and placentation. We hope that these novel findings will lead to further development of treatment for infertile patients.

人类胚胎侵入子宫内膜后，被母体血液包围。人绒毛膜促性腺激素（HCG）由胚胎滋养细胞分泌，经血液循环传到卵巢，刺激黄体功能产生孕酮，维持胚胎在子宫内着床（胚胎与母体内分泌系统的串扰）。在这些过程中，母体外周血单核细胞（PBMC）直接与胚胎的滋养外胚层接触。因此，我们认为这些PBMC可以将胚胎存在的信息传递到卵巢和子宫内膜，并有助于诱导和维持胚胎着床（通过免疫系统进行胚胎与母体之间的串扰）。基于上述新理念，本项目成功开发了一种利用自体PBMC治疗反复着床失败的不孕症患者的临床新疗法。在这个项目中，母体血小板也被证明在黄体形成和胎盘的血管生成重建中发挥重要作用。我们希望这些新发现将导致治疗不孕症患者的进一步发展。

项目成果

Intrauterine administration of autologous peripheral blood mononuclear cells promotes implantation rates in patients with repeated failure of IVF-embryo transfer

• DOI: 10.1093/humrep/del312
• 发表时间: 2006-12-01
• 期刊: HUMAN REPRODUCTION
• 影响因子: 6.1
• 作者: Yoshioka, S.;Fujiwara, H.;Fujii, S.
• 通讯作者: Fujii, S.

新しい母体-胚問の相互応答機構

一种新的母体-胚胎相互作用机制

• 发表时间: 2007
• 作者: Zeng;et. al.;藤井 治子ほか;Fujiwara H;Yoshioka S et al.;Fujii H et al.;藤原 浩;藤原 浩;藤原 浩
• 通讯作者: 藤原 浩

目で見る新しいシグナル伝達機構を介した子宮内膜機能-胚着床における制御機構;細胞間に反発力を誘導するEph-ephrinシグナルの関与-

新的视觉信号转导机制介导的子宫内膜功能 - 胚胎植入的控制机制；诱导细胞间排斥力的 Eph-ephrin 信号的参与 -

• 发表时间: 2007
• 期刊: HORMONE FRONTIER IN GYNECOLOGY 14・1
• 作者: 由良茂夫;他;Kazuyo Kakui;Shigeo Yura;Kuo-Cherng Lin;Maki Takemura;Kazuyo Kakui;Shigeo Yura;Kuo-Cherng Lin;Maki Takemura;Makoto Kawamura;Yura S et al.;Lin KC et al.;Takemura M et al.;Kawamura M et al.;Kakui K et al.;由良 茂夫;由良茂夫;藤井 治子ほか
• 通讯作者: 藤井 治子ほか

Integrin α5 is involved in fibronectin-induced human extravillous trophoblast invasion

整合素α5参与纤连蛋白诱导的人绒毛外滋养层侵袭

• 发表时间: 2007
• 期刊: J Reprod Immunol 73
• 作者: Zeng BX;Fujiwara H;Sato Y;Nishioka Y;Yamada S;Yoshioka S;Ueda M;Higuchi T;Fujii S
• 通讯作者: Fujii S

Eph-ephrin A system regulates murine blastocyst attachment and spreading

• DOI: 10.1002/dvdy.20977
• 发表时间: 2006-12-01
• 期刊: DEVELOPMENTAL DYNAMICS
• 影响因子: 2.5
• 作者: Fujii, Haruko;Tatsumi, Keiji;Fujii, Shingo
• 通讯作者: Fujii, Shingo