Induction of tissue remodeling of reproductive organs using gligl stem cell-derived factors

使用gligl干细胞衍生因子诱导生殖器官的组织重塑

• 批准号: 23659778
• 负责人: FUJIWARA Hiroshi
• 金额: $ 2.41万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659778/
• 关键词: グリア細胞; 組織再構築; 生殖臓器; 幹細胞; 上皮細胞

Co-Investigator Nishio found that when stem cells of the neural epithelium-derived glia cells were transplanted into the severed site of the cordotomized mature rat, a rapid and successful regeneration of neuronal axons beyond the lesion site was achieved during several days. Central neural tissues construct tree-dimensional structures, creating complicated neural networks, which are composed of neuroepithelium-derived neurons and glial cells. In this respect, central nervous system has been far evolved than ordinary 2-dimensional epithelial layer and therefore it is speculated that the role of the glial cells in reconstruction will throw a new insight to the process of the reconstruction in reproductive organs. Based on this concept, this study was aimed to elucidate new mechanisms of developing and/or reconstructive processes of granulosa cells, endometrial epithelial cell and trophoblasts that belong to epithelial cell-lineages. First, we screened the effects of glial cell-derived factors on the culture of granulosa cells, endometrial epithelial cells, and trophoblasts. As a result, human telomerase reverse transcriptase(h-TERT)-transfected humam endometrial epithelial cell line(provided from Dr. Kyo at Kanazawa University) promoted its intercellular connection in the presence of glial stem cells derived from EGFP-transgenic rats, showing the possibility that immature glia cell-derived soluble factors can be one of the candidates to induce reconstruction of reproductive organs and to become a useful tool in the field of reproductive medicine.

研究人员Nishio发现，当神经上皮源性神经胶质细胞的干细胞被移植到脊髓切断的成熟大鼠的切断部位时，在几天内就可以快速而成功地再生出损伤部位以外的神经元轴突。中枢神经组织构建三维结构，形成复杂的神经网络，由神经上皮源性神经元和神经胶质细胞组成。在这方面，中枢神经系统已经比普通的二维上皮层进化了很多，因此推测胶质细胞在重建中的作用将为生殖器官的重建过程提供新的见解。基于这一概念，本研究旨在阐明属于上皮细胞系的颗粒细胞、子宫内膜上皮细胞和滋养层细胞发育和/或重建过程的新机制。首先，我们筛选了胶质细胞衍生因子对颗粒细胞、子宫内膜上皮细胞和滋养层细胞培养的影响。因此，人端粒酶逆转录酶(h-TERT)转基因的人子宫内膜上皮细胞系(由金泽大学Kyo博士提供)在EGFP转基因大鼠的胶质干细胞存在下促进其细胞间连接，表明未成熟的胶质细胞衍生的可溶性因子可能是诱导生殖器官重建的候选因子之一，并成为生殖医学领域的有用工具。

项目成果

免疫細胞による着床誘導機構

免疫细胞的植入诱导机制

• 发表时间: 2011
• 作者: Hayashi T;Horiuchi A;Sano K;Gur G;Aburatani H;Ishiko O;Yaegashi N;Shiozawa T;Kanai Y;Zharhary D;Tonegawa S;Konishi I.;藤原浩
• 通讯作者: 藤原浩

Circulating immune cells can promote embryo implantation

循环免疫细胞可促进胚胎着床

• 发表时间: 2011
• 作者: 佐野健司;岩谷 舞;下条久志;浅香志穂; 的場久典;神宮邦彦;吉澤明彦;本田孝行;Fujiwara H
• 通讯作者: Fujiwara H

Circulating immune cells can promote embryo implantation.

循环免疫细胞可以促进胚胎着床。

• 发表时间: 2011
• 作者: 佐野健司;高橋千明;立石文子;神宮俊彦;大月 聡;浅香志穂;上原 剛;吉澤明彦;井手裕一郎;末木 茜;松田和之;Fujiwara H
• 通讯作者: Fujiwara H

Novel local regulatorymechanisms for human embryo attachment and invasion

人类胚胎附着和侵袭的新型局部调节机制

• 发表时间: 2011
• 作者: K. Tokita;T. Yamamoto;J. D. Boughter;Fujiwara H
• 通讯作者: Fujiwara H

EphrinA1 stimulates cell attachment and inhibits cell aggregation through the EphA receptor pathway human endometrial carcinoma-derived Ishikawa cells

EphrinA1 通过 EphA 受体途径刺激细胞附着并抑制细胞聚集人子宫内膜癌来源的 Ishikawa 细胞

• 发表时间: 2011
• 期刊: Hum Reprod
• 影响因子: 6.1
• 作者: Fujii H;Fujiwara H;Horie A;Suginami K;Sato Y;Konishi I
• 通讯作者: Konishi I