Total chemical synthesis of a redesigned enzyme, HIV-1 PR, containing an artificial tunable catalytic apparatus

重新设计的酶 HIV-1 PR 的全化学合成，包含人工可调催化装置

• 批准号: DP0346844
• 负责人: Em/Prof Paul Alewood
• 金额: $ 18.14万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0346844
• 关键词: Total; chemical; synthesis; redesigned; enzyme

The research project proposed represents a novel approach using total chemical synthesis to study the enzyme action of the HIV-1 PR, an aspartyl protease essential for the replication of AIDS virus. The redesign of the catalytic apparatus will allow us to investigate molecular aspects of its action. The synthetic polypeptide chain will be folded and characterised for the correct folded structure by NMR, and assayed for enzymatic activity. It can be expected that significant new insights into the molecular basis of the properties of the HIV-1 PR will be obtained. This will be an important contribution to biomedical research.

该研究项目提出了一种新的方法，使用全化学合成来研究HIV-1 PR的酶作用，这是一种对艾滋病病毒复制至关重要的乙酰基蛋白酶。催化装置的重新设计将使我们能够研究其作用的分子方面。将合成的多肽链折叠并通过NMR表征正确的折叠结构，并测定酶活性。可以预期，将获得对HIV-1 PR特性的分子基础的重要新见解。这将是对生物医学研究的重要贡献。