Selection of malaria protective sera useful for novel vaccine candidate discovery

选择有助于新型候选疫苗发现的疟疾保护血清

• 批准号: 19406009
• 负责人: TSUBOI Takafumi
• 金额: $ 10.73万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19406009/
• 关键词: 感染症; マラリア; ワクチン; ゲノム; タイ; バイオテクノロジー; 国際研究者交流

The individuals live in malaria endemic area who repeatedly exposed to the malaria parasite gradually develop immunity to the clinical manifestations of infection. This resistance to clinical disease is partly mediated by antibodies against the asexual blood stages of the malaria parasite. To identify such individuals harboring protective antibodies we have screened the human serum specimen useful for the future vaccine candidate discovery. After three-years surveillance in the endemic village in western Thailand, we have successfully obtained both asymptomatic sera and symptomatic patient sera as negative controls.

生活在疟疾流行区的人反复暴露于疟原虫，逐渐对感染的临床表现产生免疫力。这种对临床疾病的抵抗力部分是由针对疟原虫无性血液阶段的抗体介导的。为了鉴定这些携带保护性抗体的个体，我们筛选了可用于未来候选疫苗发现的人血清标本。在泰国西部的流行村进行了三年的监测后，我们成功地获得了无症状血清和有症状的患者血清作为阴性对照。

项目成果

Transmission-blocking activity of DNA vaccine encoding Plasmodium vivax gametocyte protein Pvs230

编码间日疟原虫配子体蛋白 Pvs230 的 DNA 疫苗的传播阻断活性

• 发表时间: 2007
• 作者: J. Okuda;T. Ramamruthy;S. Yamasaki;Tachibana M.
• 通讯作者: Tachibana M.

Novel sporozoite antigen discoverly of Plasmodium falciparum screened using human antisera.

使用人抗血清筛选恶性疟原虫的新子孢子抗原。

• 发表时间: 2007
• 作者: 松本昂;山田健太郎;高田賢蔵;西園晃;山崎伸二;Tsuboi T.
• 通讯作者: Tsuboi T.

Plasmodium falciparum rhoptry neck protein (PfRON2) expressed at both erythrocytic and pre-erythrocytic invasive parasites.

恶性疟原虫棒状颈部蛋白 (PfRON2) 在红细胞和红细胞前侵袭性寄生虫中表达。

• 发表时间: 2007
• 作者: J. Okuda;T. Ramamurthy;S. Yamasaki;Cao J.
• 通讯作者: Cao J.

Wheat germ cell-free system : A breakthrough in malaria vaccine candidate discovery

小麦胚芽无细胞系统：疟疾候选疫苗发现的突破

• 发表时间: 2008
• 作者: Feng;G・T;Kodama・X;Chen・K;Okada・T;Honda;Iriko H;Tsuboi T;Yano K;Mudeppa DG;Ghoneim A;Han ET;Tsuboi T
• 通讯作者: Tsuboi T

Erythrocyte- Binding- Like molecule and Virulence of Plasmodium yoelii

红细胞结合样分子和约氏疟原虫的毒力

• 发表时间: 2009
• 作者: Otsuki H;Kaneko O;Thongkukiatkul A;Tachibana M;Iriko H;Takeo S;Tsuboi T;Torii M
• 通讯作者: Torii M