Screening of novel malaria vaccine candidates with protective immune sera

为了鉴定新型疟疾候选疫苗，我们首先从巴布亚新几内亚疟疾队列研究中登记的儿童中获得血清样本。然后，我们建立了一个高通量检测抗原抗体反应的微型免疫筛选系统。通过使用免疫筛选系统，筛选了通过麦胚无细胞系统覆盖寄生虫基因组的三分之一的基因组广泛表达的1，900种寄生虫蛋白。作为初步筛选的结果，我们鉴定了650个免疫反应蛋白与疟疾免疫血清样品，我们以前有足够的数量。结果表明，该筛选系统将是有效的，发现新的疟疾疫苗候选人，通过使用巴布亚新几内亚血清样品。

To identify novel malaria vaccine candidates, we first obtained serum samples from the children enrolled in the malaria cohort study in Papua New Guinean. We then established a micro-scale immunoscreening system for high-throughput detection of antigen-antibody reaction. Genome-widely expressed 1,900 parasite proteins by the wheat germ cell-free system covering one-third of the parasite genome were screened by using the immunoscreening system. As a result of the preliminary screening, we identified 650 immunoreactive proteins with the malaria immune serum samples which we previously had enough amount. The result suggests that this screening system would be effective to discover novel malaria vaccine candidates by using Papua New Guinean serum samples.