Screening of novel malaria vaccine candidates with protective immune sera

为了确定新的疟疾候选疫苗，我们首先从巴布亚新几内亚参加疟疾队列研究的儿童中获得血清样本。在此基础上，建立了微尺度免疫筛选系统，用于抗原-抗体反应的高通量检测。利用免疫筛选系统筛选了覆盖三分之一小麦无生殖细胞系统的1900个全基因组广泛表达的寄生虫蛋白。初步筛选的结果是，我们在疟疾免疫血清样本中鉴定出650种免疫反应蛋白，这是我们以前有足够数量的。结果表明，该筛选系统可以有效地利用巴布亚新几内亚血清样本发现新的疟疾候选疫苗。

To identify novel malaria vaccine candidates, we first obtained serum samples from the children enrolled in the malaria cohort study in Papua New Guinean. We then established a micro-scale immunoscreening system for high-throughput detection of antigen-antibody reaction. Genome-widely expressed 1,900 parasite proteins by the wheat germ cell-free system covering one-third of the parasite genome were screened by using the immunoscreening system. As a result of the preliminary screening, we identified 650 immunoreactive proteins with the malaria immune serum samples which we previously had enough amount. The result suggests that this screening system would be effective to discover novel malaria vaccine candidates by using Papua New Guinean serum samples.