Preparation of monoclonal antibody against paclitaxel and its application for a survey and breeding of Taxus spp
Preparation of monoclonal antibody against paclitaxel and its application for a survey and breeding of Taxus spp
批准号:
19590119
负责人:
TANAKA Hiroyuki
金额:
$2.83万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2008
中文摘要
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英文摘要
キシロシルパクリタキセル(xyl-PA)をハプテンとして用い、常法により免疫原の調製、免疫感作、細胞融合を試みた結果、1種の抗PA MAbの作製に成功した。本抗体は、PAのみならずPA関連化合物であるドセタキセルに対し71%、xyl-PAに対し32%、セファロマニンに対して6.2%の交差反応性を示した。続いて、本抗体を用いて15~250 ng/mlの検量域を有する競合的システムによるEnzyme-linked immunosorbent assay(ELISA)を構築し、PA様化合物のスクリーニングに有用な手法であることを確認した。
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