The clarification of the regulatory mechanism of calcium dynamics during osteoclastogenesis

破骨细胞生成过程中钙动力学调节机制的阐明

• 批准号: 19890273
• 负责人: KURODA Yukiko
• 金额: $ 1.97万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19890273/
• 关键词: シグナル伝達; 発生・分化; 破骨細胞; カルシウムシグナル

申請者はIP3R結合タンパク質の一つであるIRBITをノックダウンすると破骨細胞分化因子RANKLによって誘導されるカルシウムオシレーションの始まるタイミングが早まる傾向があることを見いだした。この結果より、IRBITが破骨細胞分化誘導時のカルシウム動態に深く関わっている分子であることが明らかとなった。そこで、破骨細胞分化時、および未分化時にそれぞれ特異的にIRBITに結合するタンパク質のスクリーニングを行い、現在はその中の候補タンパク質の一つであるE3ユビキチンリガーゼに注目し、研究を進めている。

The applicant has a tendency to bind IP3R to the osteoclast differentiation factor RANKL and to induce it early. The results of this study show that IRBIT is a very important factor in osteoclast differentiation. When osteoclasts are differentiated, when they are not differentiated, they are bound to specific IRBIT. When they are differentiated, they are bound to IRBIT. When they are differentiated, they are bound to IRBIT. When they are differentiated, IRBIT is bound to IRBIT. When IRBIT is bound to IRBIT, they are bound to IRBIT. When IRBIT is bound to IRBIT, IRBIT is bound to IRBIT. When IRBIT is bound to IRBIT, IRBIT is bound to IRBIT, IRBIT is bound to IRBIT. When IRBIT is bound to IRBIT, IRBIT is bound to IRBIT. When IRBIT is bound to IRBIT, IRBIT is bound to IRBIT. When IRBIT is bound to IRBIT, IRBIT is bound to IRBIT. When IRBIT is bound to IRBIT, IRBIT is bound to IRBIT is bound to IRBIT. When IRBIT is bound to IRBIT is bound to IRBIT, IRBIT is bound to IRBIT is bound to IRBIT. When IRBIT is bound to IRBIT. When IRBIT is bound to IRBIT is bound to IRBIT is bound

项目成果

Abnormal taste perception in mice lacking the type 3 inositol 1,4,5-trisphosphate receptor

• DOI: 10.1074/jbc.m705641200
• 发表时间: 2007-12-21
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Hisatsune, Chihiro;Yasumatsu, Keiko;Mikoshiba, Katsuhiko
• 通讯作者: Mikoshiba, Katsuhiko

80K-H Interacts with Inositol 1,4,5-Trisphosphate (IP3) Receptors and Regulates IP3-induced Calcium Release Activity*

• DOI: 10.1074/jbc.m805828200
• 发表时间: 2009-01
• 期刊: Journal of Biological Chemistry
• 影响因子: 4.8
• 作者: Katsuhiro Kawaai;C. Hisatsune;Y. Kuroda;A. Mizutani;T. Tashiro;K. Mikoshiba

Osteoblasts induce Ca2+ oscillation-independent NFATc1 activation during osteoclastogenesis

• DOI: 10.1073/pnas.0800642105
• 发表时间: 2008-06-24
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Kuroda, Yukiko;Hisatsune, Chihiro;Mikoshiba, Katsuhiko
• 通讯作者: Mikoshiba, Katsuhiko

Phosphorylation of Homer3 by calcium/calmodulin-dependent kinase II regulates a coupling state of its target molecules in Purkinje cells

• DOI: 10.1523/jneurosci.4738-07.2008
• 发表时间: 2008-05-14
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Mizutani, Akihiro;Kuroda, Yukiko;Mikoshiba, Katsuhiko
• 通讯作者: Mikoshiba, Katsuhiko

Inositol 1,4,5-Triphosphate Receptor-binding Protein Released with Inositol 1,4,5-Triphosphate (IRBIT) Associates with Components of the mRNA 3′ Processing Machinery in a Phosphorylation-dependent Manner and Inhibits Polyadenylation

• DOI: 10.1074/jbc.m807136200
• 发表时间: 2009-04-17
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Kiefer, Helene;Mizutani, Akihiro;Mikoshiba, Katsuhiko
• 通讯作者: Mikoshiba, Katsuhiko