Elucidation of the immunological escape mechanisms and its application for cancer immunotherapy

解决免疫逃逸机制是癌症患者建立有效免疫治疗的重要问题。肺癌患者引流淋巴结和肿瘤组织中调节性T细胞积聚。调节性T细胞的耗尽导致了CTL在体外的成功诱导。通过恢复肿瘤细胞表面HLAI类分子的表达，我们可以确定可能与免疫编辑相关的抗原。最后，为了解决免疫抑制癌症患者诱导CTL的困难，我们通过转导针对肿瘤抗原的工程化T细胞受体，在体外和体内成功地产生了肿瘤细胞特异性的gd细胞。

It is the important issue to resolve immunological escape mechanisms for the establishment of effective immunotherapies in cancer patients. Regulatory T cells accumulated in draining lymph nodes and the tumor tissue in lung cancer patients. Depletion of regulatory T cells resulted in the successful induction of CTL in vitro. By restoration of HLA class I expression on tumor cells, we could identify the antigen that may be associated with immunoediting. Finally, to solve the difficulties in the induction of CTL in immunosuppresive cancer patients, we succeeded in the generation of gd cells specific for tumor cell in vitro and in vivo by transducing the engineered T cell receptor specific for tumor antigen.