Problems and their solution in the final stage of the Global Program to Eliminate Lymphatic Filariasis

在极低流行地区，利用尿液样本进行免疫诊断（尿液ELISA）可有效发现丝虫病感染者。该方法将有助于确认丝虫病的消除或发现其复发。一种新的基于尿液的视觉诊断（珠子法）也被开发出来。在没有精密设备的地区，它可以代替尿液ELISA。本文建立了一种检测蚊虫载体中丝虫DNA的LAMP方法。这些新方法将有助于发现“隐藏的”地方性疫源地，从而在消灭规划的最后阶段作出重大贡献。

An immunodiagnosis using urine samples(urine ELISA) has been found effective in finding out filaria-infected individuals in very low endemic areas. The method will be useful to confirm elimination of filariasis or detect its resurgence. A new urine-based, visual diagnosis(beads method) has also been developed. It can replace urine ELISA in areas where sophisticated equipment is not available. A LAMP method to detect filarial DNA in mosquito vectors has been developed. These new methods will help discover "hidden" endemic foci, and thus contribute greatly in the final stage of the elimination program.