Development of the analysis system for the function of glycosylation-related genes involved in embryonic lethality
Development of the analysis system for the function of glycosylation-related genes involved in embryonic lethality
批准号:
20500376
负责人:
MIYOSHI Ichiro
金额:
$3.0万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010
中文摘要
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英文摘要
We attempted to establish the analysis system for the function of Ugcg(UDP-Glucose Ceramide Glucosyltransferase) gene on the basis of the quantitative difference, from complete deficiency to overexpression, in the expression of homologue gene introduced into Ugcg-deficient ES cell on both alleles. However, stably transfected clone was note obtained. We generated Ceramide Transfer Protein (Cert) conditional knockout mice deficient in exon5 by using the Cre-LoxP system. As Cert proved to be an essential gene for mouse development and embryonic survival, this mice is a new model useful for the study of the function of glycosylation-related genes involved in embryonic lethality.
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9. 発生工学. 笠井憲雪, 吉川泰弘, 安居院高志(編)現代実験動物学168-181.
9. 发育工程。 Kenyuki Kasai、Yasuhiro Yoshikawa、Takashi Yasuin(编辑)《现代实验动物科学》168-181。
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[久野雅広, ら, 三好一郎, 三好一郎, 三好一郎]
通讯作者:
三好一郎
Rap signaling is crucial for the competence ofIL-7 response and the development of B-lineagecells
Rap 信号传导对于 IL-7 反应能力和 B 谱系细胞的发育至关重要
DOI:
10.1182/blood-2009-03-213371
发表时间:
2008
期刊:
Blood
影响因子:
20.3
作者:
[Katayama, Y., Sekai, M., Hattori, M.,Miyoshi, I., Hamazaki, Y, and Minato, N]
通讯作者:
N
ホームページ等。
主页等
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
DOI:
10.1016/j.bbrc.2011.02.071
发表时间:
2011-03-25
期刊:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子:
3.1
作者:
[Niimi, Kimie, Nishioka, Chieko, Yamashita, Tadashi]
通讯作者:
Yamashita, Tadashi
DOI:
10.1002/stem.306
发表时间:
2010-03-31
期刊:
STEM CELLS
影响因子:
5.2
作者:
[Kojima, Takuro, Hirota, Yuki, Sawamoto, Kazunobu]
通讯作者:
Sawamoto, Kazunobu
共 12 条
Establishment of model mouse susceptible to human prion; Visualization of the molecular interaction of prion protein in vivo
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批准号:11680816
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.37万
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财政年份:1999
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负责人:MIYOSHI Ichiro
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依托单位:
Basic study for establishment of the chromosome deletion model mouse
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批准号:08680902
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.73万
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财政年份:1996
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负责人:MIYOSHI Ichiro
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依托单位:
海外基金