Development of the analysis system for the function of glycosylation-related genes involved in embryonic lethality

我们尝试在Ugcg基因缺失的ES细胞中引入的同源基因从完全缺失到过表达的数量差异的基础上，建立Ugcg（udp -葡萄糖神经酰胺葡萄糖基转移酶）基因功能分析体系。然而，没有获得稳定转染的克隆。我们利用Cre-LoxP系统产生了外显子5缺失的神经酰胺转移蛋白（Cert）条件敲除小鼠。由于Cert是小鼠发育和胚胎存活的重要基因，因此该小鼠为研究糖基化相关基因参与胚胎致死性的功能提供了新的模型。

We attempted to establish the analysis system for the function of Ugcg(UDP-Glucose Ceramide Glucosyltransferase) gene on the basis of the quantitative difference, from complete deficiency to overexpression, in the expression of homologue gene introduced into Ugcg-deficient ES cell on both alleles. However, stably transfected clone was note obtained. We generated Ceramide Transfer Protein (Cert) conditional knockout mice deficient in exon5 by using the Cre-LoxP system. As Cert proved to be an essential gene for mouse development and embryonic survival, this mice is a new model useful for the study of the function of glycosylation-related genes involved in embryonic lethality.