Basis of molecular genetics of origin of simple leaf and compound leaf in vascular plant.

本研究旨在阐明维管束植物单叶和复叶调控机制的演变。我们试图以蕨类植物澳大利亚无芒草为材料建立转基因株系。然而，没有用农杆菌建立有效的转基因菌株。我们还尝试了用电穿孔法建立转基因菌株。结果表明，该方法成功地实现了愈伤组织植株的再分化。然而，目前还不能建立转基因菌株。今后拟建立蕨类植物转基因品系，明确维管束植物单叶和复叶的控制。

The aims of this study are to clarify the evolution of the controlling mechanism in the form of a simple leaf and the compound leaf of the vascular plant. We try to establish the transgenic strain using pteridophyte Asplenium australasicum. However, an effective transgenic strain was not made of establishment using Agrobacterium. We also tried to establishment of the transgenic strain by the electroporation method. As a result, it succeeded in the redifferentiation of the plant body from callus. However, the transgenic strain cannot been established yet. We want to be going to establish the transgenic strain of the pteridophyte in the future, and to clarify the control of a simple leaf and the compound leaf of vascular plant.