Development and application of the high-throughput prediction method of activity for the bioactive substances

生物活性物质活性高通量预测方法的开发及应用

• 批准号: 20590046
• 负责人: KOYAMA Junko
• 金额: $ 3.08万
• 依托单位: Kobe Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590046/
• 关键词: 分析化学; 生物活性物質; 酸化還元電位; フラボノイド; リグナン; 抗アレルギー活性; LC-MS; KU812細胞; サイクリックボルタンメトリー; ケミカルメディエーター; EBV-EA活性化抑制試験; EBV-EA活性化抑制効果; プロトベルベリン; 第1還元電位; 抗菌活性; 細胞毒性; 電子的性質

The biochemical activities (anti-promoter of tumor, anti-bacterial, anti-inflammatory, and anti-allergic activities) of quinones, flavonoids, lignans, and alkaloids were determined. Good correlation was found between the activities and the oxidation-reduction potentials by a cyclic voltammetry. It was thought that elucidating the generality of these correlations enabled a prediction of the bioactivity of compound by the physical measurement without in vitro assay. As for the result, the oxidation-reduction potential was suggested that it was a good parameter.

测定了醌类、黄酮类、木脂素和生物碱的生化活性（抗肿瘤活性、抗菌活性、抗炎活性、抗过敏活性）。循环伏安法测定了活性与氧化还原电位之间的良好相关性。人们认为，阐明这些相关性的普遍性，可以通过物理测量来预测化合物的生物活性，而无需体外测定。结果表明，氧化还原电位是一个较好的参数。

项目成果

LC-MSを用いたケミカルメディエーター類の分析と抗アレルギー活性評価への応用

使用 LC-MS 分析化学介质及其在抗过敏活性评价中的应用

• 发表时间: 2009
• 作者: 森田いずみ;小山淳子;高田秀春;森田いずみ,小山淳子,小林典裕
• 通讯作者: 森田いずみ,小山淳子,小林典裕

Development of an LC-ESI-MS/MS method for the determination of histamine: application to the quantitative measurement of histamine degranulation by KU812 cells.

• DOI: 10.1016/j.jchromb.2008.12.012
• 发表时间: 2009-01
• 期刊: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
• 作者: J. Koyama;A. Takeuchi;C. Tode;Maki Shimizu;I. Morita;M. Nobukawa;Makiko Nobukawa;N. Kobayashi-

Correlation between cytotoxic activities and reduction potentials of heterocyclic quinones.

• DOI: 10.3390/molecules15096559
• 发表时间: 2010-09-20
• 期刊: Molecules (Basel, Switzerland)
• 作者: Koyama J;Morita I;Yamori T
• 通讯作者: Yamori T

LC-APCI-MS/MSを用いたエモジンのRaji 細胞内代謝物の同定

使用 LC-APCI-MS/MS 鉴定 Raji 大黄素细胞内代谢物

• 发表时间: 2009
• 作者: 清水真希;小山淳子;竹内敦子;森田いずみ;小林典裕
• 通讯作者: 小林典裕

Casearia sylvestrisの成分研究

樟子松的成分研究

• 发表时间: 2010
• 作者: Horita Y;Takii T;Chiba T;Kuroishi R;Maeda Y;Kurono Y;Inagaki E;Nishimura K;Yamamoto Y;Abe C;Mori M;Onozaki K.;森田いずみ,小山淳子,小林典裕
• 通讯作者: 森田いずみ,小山淳子,小林典裕