Expression and biological function of a novel endogenous cholinergic polypeptide in immune cells

新型内源性胆碱能多肽在免疫细胞中的表达和生物学功能

• 批准号: 20590094
• 负责人: KAWASHIMA Koichiro
• 金额: $ 3万
• 依托单位: Musashino University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590094/
• 关键词: SLURP-1; ニコチン受容体; 気道上皮細胞; T細胞; 抗体; 喘息; 抗SLURP-1抗体; アセチルコリン; α7 nAChR; 細胞増殖; アセチルコリン産生; アセチルコリン遊離; RT-PCR; Western Blotting; 免疫組織化学; 気管支線毛上皮細胞; CHT1; α7nAChR; Western blot; マクロファージ

SLURP-1 is a novel endogenous peptide with an allosteric agonistic activity for α7 nAChR. Specific expression of SLURP-1 in the bronchial epithelial cells was discovered by an immunohistochemical procedure. SLURP-1 expression in the lung was significantly suppressed in mice with bronchial asthma. SLURP-1 modified differentiation and cholinergic activity of T cells. These findings suggest that SLURP-1 is involved in pathophysiology of asthma and regulation of immune function. We prepared two different kinds of antibodies against human type SLURP-1 in guinea pigs and rabbits in order to develop an ELISA for SLURP-1.

SLURP-1是一种新的内源性肽，对α7 nAChR具有变构激动活性。SLURP-1在支气管上皮细胞中的特异性表达通过免疫组织化学方法发现。SLURP-1在支气管哮喘小鼠肺组织中的表达明显受到抑制。SLURP-1修饰T细胞的分化和胆碱能活性。这些结果表明，SLURP-1参与哮喘的病理生理和免疫功能的调节。我们制备了两种不同类型的抗人型SLURP-1的豚鼠和兔抗体，以建立SLURP-1的ELISA。

项目成果

Expression of SLURP-1, an endogenous alpha7 nicotinic acetylcholine receptor modulator, in bronchial epithelial cells.

SLURP-1（一种内源性 α7 烟碱乙酰胆碱受体调节剂）在支气管上皮细胞中的表达。

• 发表时间: 2009
• 作者: Kawashima K;Horiguchi K;Horiguchi S;Yamashita N;Moriwaki Y;Misawa H
• 通讯作者: Misawa H

Decreased expression of SLURP-1, an endogenous alpha7 nicotinic acetylcholine receptor modulator, in the bronchus of asthmatic mice.

哮喘小鼠支气管中内源性 α7 烟碱乙酰胆碱受体调节剂 SLURP-1 的表达减少。

• 发表时间: 2010
• 作者: Narumoto O;Horiguchi K;Horiguchi S;Moriwaki Y;Takano-Ohmuro H;Shoji S;Misawa H;Yamashita N;Nagase T;Kawashima K;Yamashita N
• 通讯作者: Yamashita N

Expression of SLURP-1, an Endogenous α7 Nicotinic Acetylcholine Receptor Allosteric Ligand, in Murine Bronchial Epithelial Cells

• DOI: 10.1002/jnr.22102
• 发表时间: 2009-09-01
• 期刊: JOURNAL OF NEUROSCIENCE RESEARCH
• 影响因子: 4.2
• 作者: Horiguchi, Kazuhide;Horiguchi, Satomi;Kawashima, Koichiro
• 通讯作者: Kawashima, Koichiro

Down-regulation of secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1), an endogenous alpha7 nicotinic acetylcholine receptor modulator, in murine and human asthmatic conditions.

在小鼠和人类哮喘病中，分泌性淋巴细胞抗原 6/尿激酶型纤溶酶原激活剂受体相关肽-1 (SLURP-1) 的下调，SLURP-1 是一种内源性 α7 烟碱乙酰胆碱受体调节剂。

• 发表时间: 2010
• 期刊: Biochem Biophys Res Commun 398
• 作者: Narumoto O;Horiguchi K;Horiguchi S;Moriwaki Y;Takano-Ohmuro H;Shoji S;Misawa H;Yamashita N;Nagase T;Kawashima K;Yamashita N
• 通讯作者: Yamashita N

SLURP-1 up-regulates acetylcholine release in T cells.

SLURP-1 上调 T 细胞中乙酰胆碱的释放。

• 发表时间: 2011
• 作者: Fujii T;Takada-Takatori Y;Kawashima K
• 通讯作者: Kawashima K