Mechanisms of diseases by chromosomal translocation

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Chromosomal Translocations are one of major causes of various diseases including cancer, psychiatric disorders, and congenital anomalies. Understanding these diseases requires a technique to experimentally recapitulate diseases-specific chromosomal translocations. We improved previous methods to enable easier and quicker manipulation of mammalian chromosomes. Using this technique, we demonstrated recapitulations of two-types of cancer-causing chromosomal translocations and induction of intra-chromosomal duplication, deletion, and inversion. In addition, we applied it to measurement of distance between two genetic loci.