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Significance of miRNA in oncogenesis of soft part tumors.

miRNA 在软组织肿瘤发生中的意义。

基本信息

批准号：
20590350
负责人：
SATOH Takashi
金额：
$ 2.91万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

项目摘要

MicroRNAs (miRNA) are small non-coding RNAs whose aberrations are involved in the initiation and progression of human cancers. To seek unique miRNAs contributing to the development of osteosarcoma, we investigated the global miRNA expression profile. We focused on a commonly down-regulated miRNA, miR-127, and analyzed its relationship with the expression of its potential target, BCL6 protein. We found that 5 of 8 (63%) osteosarcoma cell lines exhibited marked down-regulation of miR-127 in comparison with the remaining cell lines and normal cultured osteoblasts. An inverse correlation between miR-127 and BCL6 protein expression was found in the osteosarcoma cell lines and primary-cultured normal osteoblasts. We demonstrated that overexpression of miR-127 induced a reduction of the BCL6 protein level, and confirmed target specificity between miR-127 and BCL6 by the luciferase reporter assay. Immunohistochemical analysis of BCL6 protein revealed that about half of the primary osteosarcomas (13/25, 52%) showed BCL6 overexpression, whereas normal osteoblasts were completely negative. The disease outcome of BCL6-positive patients was significantly worse than that of BCL6-negative patients (p<0.05). These results suggest that gain of BCL6 protein expression through the repression of miR-127 expression may be partly involved in osteosarcoma development.

微小RNA（microRNA，miRNA）是一类小的非编码RNA，其异常参与了人类肿瘤的发生和发展。为了寻找与骨肉瘤发生发展相关的独特miRNA，我们研究了miRNA的总体表达谱。我们关注一种常见的下调的miRNA，miR-127，并分析其与其潜在靶点BCL 6蛋白表达的关系。我们发现，与其余细胞系和正常培养的成骨细胞相比，8个骨肉瘤细胞系中的5个（63%）表现出miR-127的显著下调。在骨肉瘤细胞系和原代培养的正常成骨细胞中发现miR-127和BCL 6蛋白表达之间呈负相关。我们证明了miR-127的过表达诱导了BCL 6蛋白水平的降低，并通过荧光素酶报告基因测定证实了miR-127和BCL 6之间的靶特异性。BCL 6蛋白的免疫组化分析显示，约一半的原发性骨肉瘤（13/25，52%）显示BCL 6过表达，而正常成骨细胞完全阴性。BCL 6阳性患者的疾病结局明显差于BCL 6阴性患者（p<0.05）。这些结果表明，通过抑制miR-127表达而获得的BCL 6蛋白表达可能部分参与了骨肉瘤的发展。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Detection of human papillomavirus in a urothelial carcinoma mimicking urethral caruncle.

在模拟尿道肉阜的尿路上皮癌中检测人乳头瘤病毒。

DOI：
发表时间：
2010
期刊：
Int J Urol 17
影响因子：
0
作者：
Y. Tatemichi;H. Oikawa;C. Maesawa;J. Ambo;M. Sato;H. Koike;T. Sata;T. Fujioka;T. Masuda
通讯作者：
T. Masuda

Dedifferentiated liposarcoma of the retroperitoneum with osteosarcomatous component: report of two cases.

腹膜后去分化脂肪肉瘤伴骨肉瘤：二例报告。

DOI：
发表时间：
2009
期刊：
Clin Imaging 33
影响因子：
0
作者：
Toshiyasu;S. Ehara;T. Yamaguchi;J. Nishida;H. Shiraishi
通讯作者：
H. Shiraishi

Epigenetic modification is involved in aberrant expression of class III beta-tubulin, TUBB3, in ovarian cancer cells.

表观遗传修饰与卵巢癌细胞中 III 类 β-微管蛋白 TUBB3 的异常表达有关。

DOI：
发表时间：
2008
期刊：
Int J Oncol 32
影响因子：
0
作者：
Izutsu N;Maesawa C;Masuda T.;et al
通讯作者：
et al

Association of the TNF-{alpha}-C-857T polymorphism with resistance to the cholesterol-lowering effect of HMG-CoA reductase inhibitors in type 2 diabetic subjects.

TNF-{α}-C-857T 多态性与 2 型糖尿病受试者对 HMG-CoA 还原酶抑制剂降胆固醇作用的抵抗力的关联。

DOI：
发表时间：
2010
期刊：
Diabetes Care 33
影响因子：
0
作者：
T. Takahashi;K. Takahashi;M. Yamashina;C. Maesawa;T. Kajiwara;H. Taneichi;N. Takebe;Y. Kaneko;T. Masuda;J. Satoh
通讯作者：
J. Satoh

Effect of atorvastatin on microRNA 221/222 expression in endothelial progenitor cells obtained from patients with coronary artery disease