Preliminary study of the enzyme immunoassay using bacterioplankton for diagnosis of drowning

浮游细菌酶联免疫分析诊断溺水的初步研究

• 批准号: 20790463
• 负责人: KAKIZAKI Eiji
• 金额: $ 2.75万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790463/
• 关键词: 法医学; 溺死; 水棲細菌; 酵素免疫測定法

We measured bacterioplankton in blood from cadavers retrieved from the sea (n=15), near estuaries (n=8), rivers (fresh water, n=14) and from bathtubs (n=4) as well as from non-drowned victims (n=18) discovered near aquatic environments. The results suggested that the presence of numerous bacterioplankton (Vibrio and Photobacterium in seawater, Aeromonas in freshwater) in immersed cadavers could support a conclusion of death by drowning. Therefore, we investigated about enzyme immunoassay for Aeromonas hydrophila.

我们测量了从海洋（n=15）、河口附近（n=8）、河流（淡水，n=14）和浴缸（n=4）以及在水生环境附近发现的非溺水受害者（n=18）中提取的尸体血液中的浮游细菌。结果表明，浸泡尸体中大量浮游细菌（海水中的弧菌和光细菌，淡水中的气单胞菌）的存在可以支持溺水死亡的结论。因此，我们对嗜水气单胞菌的酶免疫测定方法进行了研究。

项目成果

Detection of bacterioplankton in immersed cadavers using selective agar plates.

• DOI: 10.1016/j.legalmed.2009.01.046
• 发表时间: 2009-04
• 期刊: Legal medicine
• 影响因子: 1.5
• 作者: E. Kakizaki;S. Kozawa;N. Tashiro;M. Sakai;N. Yukawa