Detection of diverse aquatic microbes in blood and organs of drowning victims by a metagenomic approach

我们使用高通量454焦磷酸测序策略来检测两名溺水受害者血液和器官中的各种水生微生物，并获得了有关各种微生物的新信息。该技术对于确定溺水受害者体内的细菌，包括蓝藻，硅藻和其他藻类等水生微生物的多样性非常有用。因此，这项技术具有很高的潜力，作为一种新的分子工具，以检查是否浸泡的受害者已经淹死，特别是当传统的硅藻测试或其他有用的方法的结果是模棱两可的。

We used a high-throughput 454-pyrosequencing strategy to detect diverse aquatic microbes in the blood and organs of two drowning victims and obtained novel information about various microbes. This technique was very useful for determining the diversity of aquatic microbes such as bacteria including cyanobacteria, diatoms and other algae in drowning victims. This technique therefore has high potential as a novel molecular tool with which to examine whether an immersed victim has drowned, especially when the results of conventional diatom testing or other useful approaches are equivocal.