Epidermal-type fatty acid binding protein (FABP5) as a potential growth regulator of oral squamous cell carcinoma.

表皮型脂肪酸结合蛋白（FABP5）作为口腔鳞状细胞癌的潜在生长调节剂。

• 批准号: 20791525
• 负责人: YAMAMOTO Noriko
• 金额: $ 2.75万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791525/
• 关键词: 口腔内癌; ノックアウトマウス; 増殖; 表皮細胞; 脂肪酸; マウス; 扁平上皮がん

To examine the functions of epidermal-type fatty acid binding protein (E-FABP) in the oral squamous cell carcinoma, we first analyzed the primary cultured keratinocytes isolated from E-FABP-deficient mice. As a result, we found that incorporation of linoleic acid into the cells was lowered by 30 % compared with wild-type cells, and that the cell-adhesion was significantly impaired in the E-FABP-deficient cells. Next, we immunohisotochemically examined the localization of E-FABP in the human SCC specimen, and found that E-FABP preferentially localized to the nuclei and cytoplasm of non-mitotic cell. The analyses of the cell line (HSC3 : human squamous cell cartinoma 3) over-expressing human E-FABP are underway especially focusing on its contribution to cell proliferation and differentiation.

为了研究表皮型脂肪酸结合蛋白（E-FABP）在口腔鳞状细胞癌中的作用，我们首先分析了从E-FABP缺陷小鼠中分离的原代培养角质形成细胞。结果，我们发现，与野生型细胞相比，向细胞中掺入亚油酸降低了30%，并且在E-FABP缺陷型细胞中细胞粘附显著受损。其次，我们用免疫组织化学方法检测了E-FABP在人SCC标本中的定位，发现E-FABP优先定位于非有丝分裂细胞的细胞核和细胞质。对过表达人E-FABP的细胞系（HSC 3：人鳞状细胞癌3）的分析正在进行中，特别关注其对细胞增殖和分化的贡献。

项目成果

Loss of Hrs in the Central Nervous System Causes Accumulation of Ubiquitinated Proteins and Neurodegeneration

• DOI: 10.2353/ajpath.2008.080684
• 发表时间: 2008-12-01
• 期刊: AMERICAN JOURNAL OF PATHOLOGY
• 影响因子: 6
• 作者: Tamai, Keiichi;Toyoshima, Masafumi;Sugamura, Kazuo
• 通讯作者: Sugamura, Kazuo

Prostaglandin F_2_α regulates cytokine production of mast cell through the receptors for prostaglandin E.

前列腺素 F_2_α 通过前列腺素 E 受体调节肥大细胞的细胞因子产生。

• 发表时间: 2008
• 期刊: Biochem. Biophys. Res. Commun. 367
• 作者: Kaneko;I
• 通讯作者: I

Fatty acid binding protein regulates TNF-α production in mast cells

脂肪酸结合蛋白调节肥大细胞中 TNF-α 的产生

• 发表时间: 2008
• 期刊: Prost Leuko Ess Fatty Acids 79
• 作者: Yamamoto;N.;Kaneko;I.;Motohashi;K.;Sakagami;H.;Adachi;Y.;Tokuda;N.;Sawada T.;Furukawa;H.;Ueyama;Y.;Fukunaga;K.;Ono;M.;Kondo;H. and Owada;Y.
• 通讯作者: Y.