Creation of artificial transcription factors for artificial gene circuits

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Artificial gene circuits is expected to provide a new way to understand various biological events controlled by feedback regulations. Towards the usage of such artificial gene circuits, artificial DNA binding proteins with various DNA binding properties, such as DNA binding affinity, selectivity, kinetics et al., would be important tools. So artificial zinc finger-type DNA binding proteins and transcription factors with various properties for DNA binding were created and examined the functions. Metal-responsive artificial DNA binding proteins and transcription factors were also successfully created.