Basic studies for a novel validation system of thyroid hormone-like activities by chemicals

化学品甲状腺激素样活性新型验证系统的基础研究

• 批准号: 21590671
• 负责人: OKABE Toshiko
• 金额: $ 3万
• 依托单位: Sagami Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590671/
• 关键词: 環境保健; 甲状腺ホルモン; 甲状腺ホルモン受容体; 緑色蛍光タンパク質; GFP; 蛍光強度; 甲状腺ホルモン応答配列; バイオアッセイ; 培養細胞; プロテアソーム阻害剤

In this study, it was demonstrated that thyroid hormone caused thyroid hormone receptor degradation specifically using the reduction of fluorescent intensities of thyroid hormone receptor-green fluorescent protein(GFP) fusion protein in cultured cells. The reduction of fluorescent intensities was dependent on the concentration of thyroid hormone in the culture medium and this reduction could be detected even in the presence of serum concentration level of thyroid hormone. From these results, it was suggested that the bioassay system using the reduction of fluorescent intensities might be more sensitive than that using transactivation by thyroid hormone receptor and thyroid hormone response element.

本研究通过降低培养细胞中甲状腺激素受体-绿色荧光蛋白(GFP)融合蛋白的荧光强度，特异性地证明了甲状腺激素引起甲状腺激素受体降解。荧光强度的下降依赖于培养液中甲状腺激素的浓度，即使在血清甲状腺激素浓度水平存在的情况下，这种下降也可以检测到。由此可见，降低荧光强度的生物测定系统可能比甲状腺激素受体和甲状腺激素反应元件反式激活的生物测定系统更灵敏。

项目成果

受容体タンパク質の分解を指標とした甲状腺ホルモン様作用評価系の開発

以受体蛋白降解为指标的甲状腺激素样效应评价系统的开发

• 发表时间: 2011
• 作者: 岡部とし子;安井奈津美
• 通讯作者: 安井奈津美