Comprehensive analysis of autoantibodies in autoimmune hepatitis using proteomics tool

在这项研究中，我们使用蛋白质组学工具分析了自身免疫性肝炎(AIH)患者的自身抗体。在自身抗体的筛选过程中，从AIH患者血清中筛选出一种自身抗体的靶抗原为磷酸烯醇式丙酮酸羧激酶(PEPCK)蛋白。抗PEPCK抗体阳性33例。3%(10/30)的AIH患者，43例。原发性胆汁性肝硬变(PBC)与AIH重叠的发生率为7%(7/16)，PBC为6%(2/30)，而健康对照组未发现。该抗体在AIH患者中检测频率高，特异性高。结论：检测抗PEPCK抗体有助于自身免疫性肝炎的诊断。

In this study, we analyzed autoantibodies in patients with autoimmune hepatitis(AIH) using proteomics tool. In the process of screening of autoantibodies, phosphoenolpyruvate carboxykinase(PEPCK) protein was identified as a target antigen of an autoantibody from serum of patients with AIH. Anti-PEPCK antibody was positive in 33. 3%(10/30) in patients with AIH, 43. 7%(7/16) in patients with primary biliary cirrhosis(PBC)-AIH overlap and 6%(2/30) in patients with PBC, but not detected in healthy control. This antibody is detected by high frequency in AIH patients and its specificity is high. In conclusion, measurement of anti-PEPCK antibody is considered to be useful for diagnosis of autoimmune hepatitis.