Comprehensive analysis of autoantibodies in autoimmune hepatitis using proteomics tool

在这项研究中，我们使用蛋白质组学工具分析自身免疫性肝炎（AIH）患者的自身抗体。在筛选自身抗体的过程中，从AIH患者血清中鉴定出磷酸烯醇丙酮酸羧激酶（PEPCK）蛋白作为自身抗体的靶抗原。抗pepck抗体阳性33例。3%（10/30）的AIH患者，43。原发性胆汁性肝硬化(PBC)-AIH重叠的患者占7%(7/16)，PBC患者占6%(2/30)，但在健康对照中未发现。该抗体在AIH患者中检测频率高，特异性高。总之，检测抗pepck抗体对自身免疫性肝炎的诊断是有价值的。

In this study, we analyzed autoantibodies in patients with autoimmune hepatitis(AIH) using proteomics tool. In the process of screening of autoantibodies, phosphoenolpyruvate carboxykinase(PEPCK) protein was identified as a target antigen of an autoantibody from serum of patients with AIH. Anti-PEPCK antibody was positive in 33. 3%(10/30) in patients with AIH, 43. 7%(7/16) in patients with primary biliary cirrhosis(PBC)-AIH overlap and 6%(2/30) in patients with PBC, but not detected in healthy control. This antibody is detected by high frequency in AIH patients and its specificity is high. In conclusion, measurement of anti-PEPCK antibody is considered to be useful for diagnosis of autoimmune hepatitis.