Multi-disciplinary analysis ob 50-100nt-sized,small RNAs Universally found in mouse cells

我们基于定制的微阵列，对最初在产生内源性反义RNA的基因位点中发现的新型大小(50-100个核苷酸)的小RNA进行了全基因组分析。用Northern杂交分析基因表达的有效性。我们建立了一种用于小RNA结构分析的核磁共振管内转录结构筛选方法。我们还使用下一代测序仪对小RNA进行了试表达分析，并对RNA序列进行了结构聚类。

We performed genome-wide analysis of novel-sized (50-100nt) small RNAs that is originally discovered in the gene loci producing endogenous antisense RNA, based on the custom-made microarrays. The expression validation was analyzed by Northern hybridization. We developed a structural screening method by the in NMR tube transcription for the structural analysis of small RNA. We also performed a trial expression analysis of small RNAs using the next generation sequencers and structural clustering of the RNA sequences.