Development of a hepatic differentiation method for embryonic stem ce l I s and i nduced p l ur i potent stem ce l I s and its application to a hybrid artificial I i ver

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In this study, we attempt to differentiate embryonic stem(ES) cells and induced pluripotent stem(iPS) cells into hepatic lineage using an original culture method in which cultured cells spontaneously form multicellular aggregates. We also developed a hybrid artificial liver device based on our culture method and demonstrated the feasibility of the device by animal experiments. ES cells and iPS cells expressed some liver specific functions after 2 weeks of the hepatic differentiation induction in the established culture method. In the animal experiments, the curative effects of the hybrid artificial liver device designed in this study were observed. In conclusion, our device containing ES cells or iPS cells may be a useful biocomponent of a liver support system.