Elucidation of the mechanism for the translation and accumulation into the endoplasmic reticulum of rice prolamine

i)对于参与脯胺mrna特异内质网定位的蛋白，选择在相应基因内检测到snp的突变系。ii) ESP1蛋白识别特异性终止密码子。iii)明确了脯氨酸在PB-I中的定位。iv)选择在分子伴侣子PDIL2-3编码基因内检测到snp的突变系。很明显，PDIL2-3促进了脯胺分子间二硫桥的形成。v)确定了脯胺积累突变的候选负责基因。

i) For the proteins participating in the localization on the specific endoplasmic reticulum of prolamine mRNAs, the mutation lines detected SNPs within the corresponding genes were selected. ii) The recognition of specific termination codon by ESP1 protein was suggested. iii) It was clear the localization of prolamines in PB-I. iv) The mutation lines detected the SNPs within the gene encoding the molecular chaperon PDIL2-3 were selected. It was clear that PDIL2-3 promoted the formation of disulfide-bridge between prolamine molecules. v) The candidate responsible genes of the mutations for prolamine accumulation were identified.