权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Role of bacteriophage communities in paddy soils and their gene diversity and uniqueness

水稻土中噬菌体群落的作用及其基因多样性和独特性

基本信息

批准号：
21380046
负责人：
KIMURA Makoto
金额：
$ 11.23万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

The role of bacteriophage(phage) communities in paddy soils was studied from the phage contribution to bio-element cycling in this study, and the frequency of phage infection to their hosts and the carbon flow of plant residue to phage DNA were evaluated. Fresh and dried^13C-labelled callus cells were used as the model materials of root cap cells and plant residue, respectively. The gene diversity and uniqueness of phage communities in soils were examined from the phage g23, g20 and psbA sequences in comparison with those in marine and freshwater environments. The frequency of virulent phage infection to bacteria in soils was markedly higher than those reported from marine and freshwater environments. The recovery of 13C-labelled g23 and g20 clones indicated that the phage communities are important in bio-element cycling as the drivers of the microbial loop. The diversity of g23 clones was significantly higher than those in marine and freshwater environments, and their sequences were mostly soil-specific and different from those of marine and freshwater origins. In addition, it was known that g20 and psbA gene sequences in the floodwater of paddy fields were more similar to the sequences in the freshwater environment than to those in the marine environments and that g20 sequences in paddy field soils were different from those not only of marine and freshwater origins but also in the floodwaters overlying.

本研究从噬菌体对生物元素循环的贡献出发，研究了水稻土中噬菌体群落的作用，评价了噬菌体感染宿主的频率和植物残体对噬菌体DNA的碳流。分别以新鲜和干燥的~(13)C标记的愈伤组织细胞作为根冠细胞和植物残渣的模型材料。从噬菌体G23、G20和PSBA序列分析了土壤中噬菌体群落的基因多样性和唯一性，并与海水和淡水环境中的噬菌体群落进行了比较。土壤中细菌的毒力噬菌体感染频率明显高于海洋和淡水环境中的报道。13C标记的G23和G20克隆的恢复表明，噬菌体群落作为微生物环的驱动因素在生物元素循环中起着重要作用。G23克隆的多样性显著高于海洋和淡水环境中的克隆，并且它们的序列大多是土壤特有的，不同于海洋和淡水来源的序列。此外，已知稻田洪水中的G20和PSBA基因序列与淡水环境中的序列比与海洋环境中的序列更相似，稻田土壤中的G20序列不仅与海洋和淡水来源的序列不同，而且与上覆洪水的序列也不同。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Bacterial communities incorporating carbon of root cap cells under upland soil conditions

高地土壤条件下吸收根冠细胞碳的细菌群落

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Yong Li;Chol Gyu Lee;Takeshi Watanabe;Jun Murase;Susumu Asakawa;and Makoto Kimura
通讯作者：
and Makoto Kimura

Ecology of viruses in rice fields

稻田病毒生态学

DOI：
发表时间：
2010
期刊：
影响因子：
0
作者：
Makoto Kimura;Guanghua Wang;Natsuko Nakayama;and Susumu Asakawa
通讯作者：
and Susumu Asakawa

ファージ感染が土壌細菌の計数に及ぼす影響

噬菌体感染对土壤细菌计数的影响

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
高橋龍;浅川晋;木村偉人
通讯作者：
木村偉人

Changes in major capsid genes (g23) of T4-type bacteriophages with soil depth in two Japanese rice fields

DOI：
10.1007/s00374-009-0362-2
发表时间：
2009-02
期刊：
Biology and Fertility of Soils
影响因子：
6.5
作者：
Guanghua Wang;J. Murase;K. Taki;Yoshinori Ohashi;N. Yoshikawa;S. Asakawa;M. Kimura
通讯作者：
Guanghua Wang;J. Murase;K. Taki;Yoshinori Ohashi;N. Yoshikawa;S. Asakawa;M. Kimura

Low diversity of T4-type bacteriophages in applied rice straw, plant residues and rice roots in Japanese rice soils: Estimation from major capsid gene (g23) composition