Studies on physiological roles of ER-60 by tissue-specific gene targeting analysis

在这个项目中，我们发现一些膜糖蛋白的折叠效率受到肝脏特异性ER-60基因敲除的影响。结果表明，长期饲喂高脂饲料可诱发ER-60基因敲除小鼠的内质网应激。我们试图通过脑特异性ER-60基因敲除来证实ER-60对淀粉样β多肽毒性的保护作用。此外，我们还成功地对ER-60的BB‘片段与β-淀粉样肽的复合体进行了X射线结构分析。

In this project, we found that the folding efficiency of some membrane glycoproteins was impaired by liver-specific knockout of ER-60. It was demonstrated that ER stress might be easily induced in ER-60 knockout mice by long feeding of high-fat diet. We attempted to confirm the protective effects of ER-60 against toxicity of amyloid beta peptides by brain-specific ER-60 knockout. In addition, we succeeded in X-ray structural analysis of a complex with the bb' fragment of ER-60 andβ-amyloid peptide.