What dietary supplementation can acclimate broiler chickens to heat stress?

哪些膳食补充剂可以使肉鸡适应热应激？

• 批准号: 21380165
• 负责人: TOYOMIZU Masaaki
• 金额: $ 11.48万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380165/
• 关键词: 暑熱ストレス; 地球温暖化対策; ミトコンドリア; 抗酸化飼料; ニワトリ; 活性酸素

The objective of this study was to find a possible method to improve growth performance of broiler chickens exposed to chronic heat stress. 1)Mitochondrial ROS production for control group showed little changes during the experimental periods, whereas that for the heat-stressed group (34°C)was increased after 3, 5 and 9 d of heat exposure and returned to original levels at 14d. 2)Myoblasts isolated from the pectoralissuperficialis muscles of 0-1 day old chicks were cultured and exposed to 41°C for 6 h, resulting in increased ROS production and reduced protein content of the cells. These changes were attenuated by pretreatment ofan antioxidant, Tempol for 1 h. 3)Supplementation of glutamine and glutamate to basal diets slightly but definitely alleviated decreases in body weight gain, food intake and, and ratio of the duodenal villus height to crypt depth of heat-stressed broiler chickens. Further biochemical studies including modifications of intestinal function are required to establish a method for alleviation of the stress.

本研究旨在寻找一种改善慢性热应激肉鸡生长性能的方法。1)对照组线粒体活性氧的产生在整个实验期间变化不大，而热应激组（34°C）在热暴露3、5和9 d后线粒体活性氧的产生增加，并在14 d时恢复到原始水平。2)培养从0-1日龄雏鸡的胸肌表面肌分离的成肌细胞，并将其暴露于41°C 6小时，导致细胞的ROS产生增加和蛋白质含量降低。抗氧化剂Tempol预处理1h可减弱上述变化。3)在基础日粮中添加谷氨酰胺和谷氨酸，可明显缓解热应激肉鸡增重、采食量和十二指肠绒毛高度与隐窝深度比值的下降。需要进一步的生化研究，包括肠道功能的修改，以建立一种方法来缓解压力。

项目成果

急性暑熱にともなう鶏骨格筋におけるROS過剰産生機構の解明

阐明急性热导致鸡骨骼肌ROS过量产生的机制

• 发表时间: 2010
• 期刊: 家畜栄養生理研究会報
• 作者: Ueda;W.;M. Tohno;T. Shimazu;H. Fujie;H. Aso;Y. Kawai;M. Numasaki;T. Saito;H. Kitazawa;工藤亮治・増本隆夫・吉田武郎・堀川直紀;小松崎将一;杉山修一;喜久里基
• 通讯作者: 喜久里基

Mitochondrial membrane potential decrease caused by loss of PINK1 isnot due to proton leak, not to respiratory chain defects

PINK1 缺失引起的线粒体膜电位降低不是由于质子泄漏，也不是由于呼吸链缺陷

• 发表时间: 2010
• 期刊: Neurobiol.Dis.
• 作者: Amo;T.;Sato;S.;Saiki;S.;Wolf;A.M.;Toyomizu;M.;Gautier;C.A.;Shen;L.;Ohta;S.;Hattori;N.
• 通讯作者: N.

Meat-type chickens have a higher efficiency of mitochondrial oxidative phosphorylation than laying-type chickens

• DOI: 10.1016/j.cbpa.2011.01.020
• 发表时间: 2011-05-01
• 期刊: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
• 影响因子: 2.3
• 作者: Toyomizu, Masaaki;Kikusato, Motoi;Amo, Taku
• 通讯作者: Amo, Taku

鶏骨格筋細胞を用いた暑熱ストレスモデルの構築

鸡骨骼肌细胞热应激模型的构建

• 发表时间: 2012
• 作者: Escudero E;et al;小松崎将一;吉田隼巳
• 通讯作者: 吉田隼巳

Mechanistic analysis of mitochondrial EOS production in chicken skeletal muscle under acute heat stress conditions

急性热应激条件下鸡骨骼肌线粒体EOS产生机制分析

• 发表时间: 2010
• 作者: Iwasaki K;Miwa Y;Haneda M;Kobayashi T.;喜久里基
• 通讯作者: 喜久里基