Isolation and characterization of portal branch ligation-stimulated liver-specific stem cells

我们研究了在门静脉分支结扎的肝脏模型中分离肝祖细胞的可能性，而不用任何化学药物。从门静脉分支结扎或未结扎的肝叶制备非实质细胞组分，种植到涂有层粘连蛋白的平板上。大多数细胞死亡，但一小部分细胞能够增殖。有限稀释法将这些增殖细胞克隆为门静脉分支结扎刺激的肝细胞(PBLHCs)。PBLHC具有干细胞特性，可分化为肝细胞和胆管细胞。结论：成功地从门静脉结扎小鼠肝叶分离出双能肝祖细胞克隆，为门静脉结扎诱导肝祖细胞的临床应用提供了可能。

We investigated the possibility of isolating hepatic progenitor cells in a portal branch-ligated liver model without administration of any chemical agents. A non-parenchymal cell fraction was prepared from the portal branch-ligated or non-ligated lobe, and seeded onto plates coated with laminin. Most of the cells died, but a small number were able to proliferate. These proliferating cells were cloned as portal branch ligation-stimulated hepatic cells(PBLHCs) by the limiting dilution method. The PBLHCs had a property of stem cells, and differentiated into both hepatocyates and cholangiocytes. In conclusion, successful isolation of bipotent hepatic progenitor cell clones, PBLHCs, from the portal branch-ligated liver lobes of mice provides the possibility of future clinical application of portal vein ligation to induce hepatic progenitor cells.