Isolation and characterization of portal branch ligation-stimulated liver-specific stem cells

门静脉分支结扎刺激的肝脏特异性干细胞的分离和表征

• 批准号: 21390375
• 负责人: MIYAGAWA Shinichi
• 金额: $ 11.48万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390375/
• 关键词: 肝臓外科学; 再生医学; 門脈結紮(塞栓); 肝再生; 組織幹細胞

We investigated the possibility of isolating hepatic progenitor cells in a portal branch-ligated liver model without administration of any chemical agents. A non-parenchymal cell fraction was prepared from the portal branch-ligated or non-ligated lobe, and seeded onto plates coated with laminin. Most of the cells died, but a small number were able to proliferate. These proliferating cells were cloned as portal branch ligation-stimulated hepatic cells(PBLHCs) by the limiting dilution method. The PBLHCs had a property of stem cells, and differentiated into both hepatocyates and cholangiocytes. In conclusion, successful isolation of bipotent hepatic progenitor cell clones, PBLHCs, from the portal branch-ligated liver lobes of mice provides the possibility of future clinical application of portal vein ligation to induce hepatic progenitor cells.

我们研究了在门静脉分支结扎肝脏模型中分离肝祖细胞而不使用任何化学试剂的可能性。从门静脉分支结扎或未结扎的叶制备非实质细胞级分，并将其接种到涂有层粘连蛋白的平板上。大多数细胞死亡，但少数细胞能够增殖。通过有限稀释法将这些增殖细胞克隆为门静脉分支结扎刺激肝细胞（PBLHC）。 PBLHC具有干细胞的特性，可分化为肝细胞和胆管细胞。总之，从小鼠门静脉分支结扎肝叶中成功分离双能肝祖细胞克隆（PBLHC）为未来门静脉结扎诱导肝祖细胞的临床应用提供了可能性。

项目成果

Potential Feasibility of Early Bone Marrow Cell Injection Into the Spleen for Creating Functional Hepatocytes

• DOI: 10.1097/tp.0b013e31819e0528
• 发表时间: 2009-04
• 期刊: Transplantation
• 影响因子: 6.2
• 作者: R. Misawa;J. Soeda;H. Ise;Masafumi Takahashi;K. Kubota;A. Mita;Takenari Nakata;S. Miyagawa

COP35, a Cholangiocarcinoma-Binding Oligopeptide, Interacts with the Clathrin Heavy Chain Accompanied by GRP78

• DOI: 10.1158/1541-7786.mcr-10-0470
• 发表时间: 2011-06-01
• 期刊: MOLECULAR CANCER RESEARCH
• 影响因子: 5.2
• 作者: Kitahara, Hiroe;Masumoto, Junya;Miyagawa, Shinichi
• 通讯作者: Miyagawa, Shinichi

An excellent non-viral procedure to induce liver-to-pancreas reprogramming to complement post-operative pancreatic endocrine dysfunction.

一种出色的非病毒程序，可诱导肝脏至胰腺重新编程，以补充术后胰腺内分泌功能障碍。

• 发表时间: 2010
• 作者: Kobayashi A;Miyagawa S;Miwa S;Nakata T.;中田岳成;中田岳成;Hiroaki Motoyama;Hiroaki Motoyama
• 通讯作者: Hiroaki Motoyama

Adrenomedullin in sinusoidal endothelial cells play protective roles against cold injury of liver

• DOI: 10.1016/j.peptides.2010.01.011
• 发表时间: 2010-05
• 期刊: Peptides
• 影响因子: 3
• 作者: Nobuyoshi Iinuma;T. Sakurai;Akiko Kamiyoshi;Yuka Ichikawa-Shindo;Takuma Arai;T. Yoshizawa;T. Koyama;Ryuichi Uetake;Hisaka Kawate;S. Muto;Y. Tagawa;S. Miyagawa;T. Shindo

Clinicopathological features of hepatitis C virus disease after living donor liver transplantation: relationship with in situ hybridisation data.

活体肝移植后丙型肝炎病毒病的临床病理特征：与原位杂交数据的关系。

• DOI: 10.1097/pat.0b013e32834317ed
• 发表时间: 2011
• 期刊: Pathology
• 影响因子: 4.5
• 作者: Masuda Y;Nakazawa Y;Matsuda K;Sano K;Mita A;Ohno Y;Urata K;Ikegami T;Miwa S;Miyagawa S.
• 通讯作者: Miyagawa S.