Tyrosine kinase gene mutation and chemotherapy sensitivity in lung cancers.

评估肺癌辅助化疗患者的ERCC1 C8092A和codon118C/T基因多态性状态。我们研究了NRF2基因在肺鳞状细胞癌中的突变状态，并与拷贝数、MRP3（多药耐药相关蛋白3）表达及其他临床病理背景进行了比较。新一代测序技术在肺癌中发现了新的KIF5B/RET易位。转染KIF5B/RET的细胞对抗RET药物敏感。我们建立了荧光原位杂交（FISH）法检测KIF5B/RET输血基因。

ERCC1 C8092A and codon118C/T gene polymorphism statuses were evaluated for lung cancer patients who recieved adjuvant chemotherapy. We have investigated NRF2 gene mutation statuses in lung squamous cell carcinomas and compared with copy number, MRP3(multi-drug resistance-associated protein 3) expression and other clinico-pathological backgrounds. Novel KIF5B/RET translocation was found in lung cancer using a next-generation sequencing assay. KIF5B/RET transfected cells were sensitive to anti-RET drugs. We have developed fluorescence in situ hybridization(FISH) assay to detect KIF5B/RET transfusion gene.