Tyrosine kinase gene mutation and chemotherapy sensitivity in lung cancers.

肺癌中酪氨酸激酶基因突变和化疗敏感性。

• 批准号: 21390394
• 负责人: FUJII Yoshitaka
• 金额: $ 11.56万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390394/
• 关键词: 肺癌; ERCC1; NRF2; KIF5B/RET; RET阻害剤; FISH; MRP3; EGFR; KIF5B; RET; 遺伝子転座; FGFR1; コピー数; Kras; AKT2; MEK1; NFE2L2; 扁平上皮癌; βチュブリン; MCL1; BCL2L1

ERCC1 C8092A and codon118C/T gene polymorphism statuses were evaluated for lung cancer patients who recieved adjuvant chemotherapy. We have investigated NRF2 gene mutation statuses in lung squamous cell carcinomas and compared with copy number, MRP3(multi-drug resistance-associated protein 3) expression and other clinico-pathological backgrounds. Novel KIF5B/RET translocation was found in lung cancer using a next-generation sequencing assay. KIF5B/RET transfected cells were sensitive to anti-RET drugs. We have developed fluorescence in situ hybridization(FISH) assay to detect KIF5B/RET transfusion gene.

对接受辅助化疗的肺癌患者进行ERCC 1 C8092 A和codon 118 C/T基因多态性检测。我们研究了NRF 2基因在肺鳞癌中的突变状态，并与拷贝数、多药耐药相关蛋白3（MRP 3）表达和其他临床病理背景进行了比较。使用下一代测序分析在肺癌中发现了新的KIF 5 B/RET易位。KIF 5 B/RET转染细胞对抗RET药物敏感。我们建立了检测KIF 5 B/RET基因的荧光原位杂交（FISH）方法。

项目成果

Thoracic and cardiovascular surgery in Japan during 2008 : Annual report by The Japanese Association for Thoracic Surgery

2008年日本的胸外科和心血管外科：日本胸外科协会年度报告

• 发表时间: 2010
• 期刊: Gen Thorac Cardiovasc Surg
• 作者: Sakata R;Fujii Y;Kuwano H
• 通讯作者: Kuwano H

MEK1 and AKT2 Mutations in Japanese Lung Cancer

• DOI: 10.1097/jto.0b013e3181d35236
• 发表时间: 2010-05
• 期刊: Journal of Thoracic Oncology
• 影响因子: 20.4
• 作者: H. Sasaki;Y. Hikosaka;O. Kawano;S. Moriyama;M. Yano;Y. Fujii

Evaluation of Kras Gene Mutation and Copy Number Gain in Non-small Cell Lung Cancer

• DOI: 10.1097/jto.0b013e31820594f0
• 发表时间: 2011-01-01
• 期刊: JOURNAL OF THORACIC ONCOLOGY
• 影响因子: 20.4
• 作者: Sasaki, Hidefumi;Hikosaka, Yu;Fujii, Yoshitaka
• 通讯作者: Fujii, Yoshitaka

Epidermal growth factor receptor gene amplification in surgical resected Japanese lung cancer

• DOI: 10.1016/j.lungcan.2008.10.005
• 发表时间: 2009-06-01
• 期刊: LUNG CANCER
• 影响因子: 5.3
• 作者: Sasaki, Hidefumi;Shimizu, Shigeki;Fujii, Yoshitaka
• 通讯作者: Fujii, Yoshitaka

NFE2L2 gene mutation in Japanese lung cancer patients

日本肺癌患者NFE2L2基因突变

• 发表时间: 2010
• 作者: Sasaki H;Hikosaka Y;Kawano O;Okuda K;Moriyama S;Yano M;Fujii Y
• 通讯作者: Fujii Y