Antimicrobial and osteoconductivity of titanium implant surface.

钛种植体表面的抗菌性和骨传导性。

• 批准号: 21390527
• 负责人: MIYAZAKI Takashi
• 金额: $ 10.32万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390527/
• 关键词: チタン; インプラント; 抗菌; 表面分析; 細胞

Initial adhesion of micro-organisms onto such orthopedic and dental implant surfaces is an unavoidable event that triggers biofilm formation. Biofilm formation on implanted medical devices is a major complication leading to potential surgical failure. When titanium is anodized by discharge in NaCl solution, both antimicrobial activity and osteoconductivity are conferred. The viability of adherent micro-organisms and cells on antimicrobial titanium remains uncertain. We hypothesized that a thin peroxidation barrier would efficiently destroy adherent bacteria, whereas adherent osteoblastic cells would be viable, since these cells adhere to the surface indirectly though serum proteins. The efficacy of antimicrobial titanium appears to be based on peroxidation, since peroxidation products were detected in parallel with the destruction of bacterial cell-surface structures. The peroxidation effect of antimicrobial titanium was confined to the surface within narrow limits. The viability of osteoblastic cells on the surface was strongly dependent on the presence of serum protein, whereas that of adherent Streptococcus mutans was not affected by the presence of serum proteins. Therefore, differences in the adherent systems used by bacteria and osteoblastic cells are important determinants of their viability on antimicrobial titanium.

微生物最初粘附在此类整形外科和牙科植入物表面上是不可避免的事件，会引发生物膜的形成。植入医疗器械上生物膜的形成是导致潜在手术失败的主要并发症。当钛通过在氯化钠溶液中放电而被阳极氧化时，具有抗菌活性和骨传导性。抗菌钛上附着的微生物和细胞的生存能力仍不确定。我们假设薄的过氧化屏障将有效地破坏粘附的细菌，而粘附的成骨细胞将是可行的，因为这些细胞通过血清蛋白间接粘附到表面。抗菌钛的功效似乎基于过氧化，因为在破坏细菌细胞表面结构的同时检测到过氧化产物。抗菌钛的过氧化作用仅限于表面，范围很窄。表面成骨细胞的活力强烈依赖于血清蛋白的存在，而粘附的变形链球菌的活力则不受血清蛋白存在的影响。因此，细菌和成骨细胞使用的粘附系统的差异是它们对抗菌钛的活力的重要决定因素。

项目成果

Antimicrobial and osteogenic properties of a hydrophilic-modified nanoscale hydroxyapatite coating on titanium.

• DOI: 10.1016/j.nano.2011.07.001
• 发表时间: 2012-04
• 期刊: Nanomedicine : nanotechnology, biology, and medicine
• 作者: A. Murakami;T. Arimoto;D. Suzuki;Misato Iwai-Yoshida;Fukunaga Otsuka;Y. Shibata;T. Igarashi;R. Kamijo;T. Miyazaki

The characteristics of in vitro biological activity of titanium surfaces anodically oxidized in chloride solutions.

• DOI: 10.1016/j.biomaterials.2010.07.098
• 发表时间: 2010-11
• 期刊: Biomaterials
• 影响因子: 14
• 作者: Y. Shibata;D. Suzuki;S. Omori;R. Tanaka;A. Murakami;Y. Kataoka;Kazuyoshi Baba;R. Kamijo

チタンインプラントの高機能化を目指した表面改質

旨在提高钛植入物功能的表面改性

• 发表时间: 2010
• 作者: 柴田陽;宮崎隆
• 通讯作者: 宮崎隆

Antimicrobial Activity and Osteoblastic Responses to Anodically Oxidized Titanium Surfaces.

对阳极氧化钛表面的抗菌活性和成骨细胞反应。

• 发表时间: 2011
• 作者: Akiyama T;et al;Tanaka R
• 通讯作者: Tanaka R

Role of the chloride formed on anodized titanium surfaces against an oral microorganism.

阳极氧化钛表面形成的氯化物对抗口腔微生物的作用。

• 发表时间: 2010
• 期刊: J Biomater Appl 24(in press)
• 作者: Deng J.Y.;Arimoto T.;Shibata Y.;Omori S.;Miyazaki T.
• 通讯作者: Miyazaki T.