Effect of exosome-associated gangliosides on the assembly of amyloid-β protein

外泌体相关神经节苷脂对β淀粉样蛋白组装的影响

• 批准号: 21770128
• 负责人: YUYAMA Kohei
• 金额: $ 1.66万
• 依托单位: Hokkaido University (2010)National Institute for Longevity Sciences,NCGG (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770128/
• 关键词: 糖質; ガングリオシド; アルツハイマー病; エクソソーム

Alzheimer's disease is pathologically featured by extraneuronal deposition of amyloid fibrils, which are composed of amyloid-β protein (Aβ). However, it still remains to be clarified how soluble monomeric Aβ assemble into fibrils. In the present study, we found that ganglioside GM1 is release from mouse primary cortical neurons, in the association with exosome. Furthermore, the exosome-associated GM1 accelerated the assembly of synthetic Aβ in vitro.

阿尔茨海默病的病理特征是淀粉样蛋白原纤维在神经元外沉积，淀粉样蛋白原纤维由淀粉样蛋白-β (Aβ)组成。然而，可溶性单体Aβ如何组装成原纤维仍不清楚。在本研究中，我们发现神经节苷脂GM1从小鼠初级皮质神经元中释放，并与外泌体相关。此外，外泌体相关的GM1加速了体外合成Aβ的组装。

项目成果

Sphingomyelin accumulation provides a favorable milieu for GM1 ganglioside-induced assembly of amyloid β-protein

• DOI: 10.1016/j.neulet.2010.06.080
• 发表时间: 2010-09
• 期刊: Neuroscience Letters
• 影响因子: 2.5
• 作者: K. Yuyama;K. Yanagisawa

Endocytic pathway perturbation as a putative cause of GM1-ganglioside-induced amyloid fibril formation in Alzheimer's disease.

内吞途径扰动是阿尔茨海默病中 GM1-神经节苷脂诱导淀粉样原纤维形成的假定原因。

• 发表时间: 2010
• 作者: Yuyama K.;Yanagisawa K.;Kohei Yuyama
• 通讯作者: Kohei Yuyama

Ganglioside GD3 monoclonal antibody-induced paxillin tyrosine phosphorylation and filamentous actin assembly in cerebellar growth cones.

神经节苷脂 GD3 单克隆抗体诱导小脑生长锥中桩蛋白酪氨酸磷酸化和丝状肌动蛋白组装。

• DOI: 10.1111/j.1471-4159.2010.07071.x
• 发表时间: 2011
• 期刊: J.Neurochem.
• 作者: Yuyama K;Sekino-Suzuki N;Yamamoto N;Kasahara K
• 通讯作者: Kasahara K

エンドサイトーシス障害におけるGM1ガングリオシド依存性アミロイドβ蛋白質重合

内吞性疾病中 GM1 神经节苷脂依赖性淀粉样蛋白-β 蛋白聚合

• 发表时间: 2009
• 作者: Yuyama K.;Yanagisawa K.;Kohei Yuyama;湯山耕平
• 通讯作者: 湯山耕平