Exfoliation of gastric pit-parietal cells into the gastric lumen associated with a stimulation of acid-secretion observed in isolated rat gastric mucosa model.

在离体大鼠胃粘膜模型中观察到胃凹壁细胞脱落到胃腔中与酸分泌刺激相关。

• 批准号: 21790186
• 负责人: TOYOSHIMA Fumiyo
• 金额: $ 2.66万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790186/
• 关键词: 細胞・組織; 胃粘膜; 壁細胞; 超微形態; オートファジー

Gastric parietal cells of the gastric gland(especially at the pit) are highly differentiated cells responsible for the gastric hydrochloric acid secretion into the lumen of the stomach. We have recently noted that a number of pit-parietal cells were exfoliated into the lumen of isolated rat gastric mucosa model, and applied the cryo-techniques to elucidate the fine structure and histochemical characteristics in the process of cell exfoliation. Quantitative analysis verified a time-dependent increase in the number of cell exfoliation in the gastric mucosa isolated under acid-stimulation by histamine treatment compared with under acid-inhibition by H2-antagonist or proton pump inhibitor(PPI) treatment. Furthermore, an in vitro preliminary model of gastric erosion has been established with NSAIDs(Non-Steroidal Anti-Inflammatory Drugs) treatment. Acceleration of the cell exfoliations may result in the breakdown of gastric epithelial continuities. Therefore the sealing of the basal spot of exfoliated pit-parietal cells by neighboring mucous cells seems profitable to prevent an epithelial deficiency leading to gastric erosion and ulcer.

胃腺的胃壁细胞(尤其是小凹)是高度分化的细胞，负责将胃酸分泌到胃腔内。我们最近注意到，在大鼠胃粘膜分离模型中，有大量的壁窝细胞脱落到管腔内，并用冷冻技术阐明了细胞脱落过程中的精细结构和组织化学特征。定量分析证实，与H2拮抗剂或质子泵抑制剂(PPI)处理的酸抑制相比，组胺处理的酸刺激下分离的胃粘膜细胞脱落数量随时间而增加。此外，还建立了非类固醇抗炎药(NSAIDs)治疗的胃黏膜糜烂体外模型。细胞加速脱落可能导致胃上皮细胞连续性的破坏。因此，用邻近的粘液细胞封闭脱落的小窝-壁细胞的基底点，似乎有利于防止导致胃溃疡和胃黏膜上皮的缺失。

项目成果

高圧凍結技法による胃粘膜の新たな超微形態研究

高压冷冻技术胃粘膜超形态学新研究

• 发表时间: 2011
• 作者: 澤口朗;豊嶋典世;菅沼龍夫
• 通讯作者: 菅沼龍夫

凍結技法を用いたラット胃小窩壁細胞の分化における糖鎖構造(TF抗原)の発現

冷冻技术诱导大鼠胃凹壁细胞分化过程中碳水化合物结构（TF抗原）的表达

• 发表时间: 2011
• 作者: 豊嶋(青山)典世;杉原ひとみ;高橋伸育;井手惣幸;澤口朗
• 通讯作者: 澤口朗

凍結技法と単離胃粘膜モデルを応用した胃酸分泌関連疾患の病態解析

利用冷冻技术和离体胃粘膜模型对胃酸分泌相关疾病进行病理分析

• 发表时间: 2012
• 作者: 豊嶋典世;髙橋伸育;井手惣幸;澤口朗
• 通讯作者: 澤口朗

Functional Transformation of Gastric Parietal Cells and Intracellular Trafficking of Ion Channels/Transporters in the Apical Canalicular Membrane Associated with Acid Secretion

• DOI: 10.1248/bpb.34.813
• 发表时间: 2011-06-01
• 期刊: BIOLOGICAL & PHARMACEUTICAL BULLETIN
• 影响因子: 2
• 作者: Aoyama, Fumiyo;Sawaguchi, Akira
• 通讯作者: Sawaguchi, Akira

高圧凍結技法による胃粘膜の超微形態研究

高压冷冻技术对胃粘膜超微结构的研究

• 发表时间: 2010
• 期刊: 顕微鏡 45(2)
• 作者: 澤口朗;豊嶋典世
• 通讯作者: 豊嶋典世